PARK7 / DJ-1 Antibody (Antigen Affinity Purified)

PARK7 / DJ-1 Antibody ( Antigen Affinity Purified ) Datasheet

PARK7 / DJ-1 Antibody Product Information

• Immunogen: Recombinant Human PARK7 / DJ-1 protein (Catalog#12484-H08E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human PARK7 / DJ-1 (rh PARK7 / DJ-1; Catalog#12484-H08E; Q99497-1; Met 1-Asp 189). PARK7 / DJ-1 specific IgG was purified by Human PARK7 / DJ-1 affinity chromatography.

PARK7 / DJ-1 Antibody Usage Guide

• Specificity: Human PARK7 / DJ-1
• Immunochemistry: IHC-P: 0.05-1 μg/mL

  

  Immunochemical staining of human PARK7 in human brain with rabbit polyclonal antibody (0.2 μg/mL, formalin-fixed paraffin embedded sections).

  Immunochemical staining of human PARK7 in human thyroid gland with rabbit polyclonal antibody (0.2 μg/mL, formalin-fixed paraffin embedded sections).

• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human PARK7 in WB. Using a DAB detection system, the detection limit for Human PARK7 is approximately 2 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human PARK7. The detection limit for Human PARK7 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

PARK7 / DJ-1 Antibody Background

Parkinson's disease locus DJ-1 (PARK7) belongs to the peptidase C56 family of proteins, which acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. DJ-1/p53 interactions contribute to apoptosis resistance in clonal myeloid cells and may serve as a prognostic marker in patients with myelodysplastic syndromes (MDS). Defects in the PARK7 gene are the cause of autosomal recessive early-onset Parkinson's disease 7. Originally identified as an oncogene, DJ-1 is a ubiquitous redox-responsive cytoprotective protein with diverse functions. In addition to cell-autonomous neuroprotective roles, DJ-1 may act in a transcellular manner, being up-regulated in reactive astrocytes in chronic neurodegenerative diseases as well as in stroke. Thus, DJ-1, particularly in its oxidized form, has been recognized as a biomarker for cancer and neurodegenerative diseases.

References

1. Niki Takeshi. et al., 2003, Mol. Cancer Res. 1 (4): 247-61.
2. Takahashi, K. et al., 2001, J. Biol. Chem. 276 (40): 37556-63.
3. Marcondes AM. et al., 2010, Blood. 115 (10): 1993-2002.
4. Kahle PJ. et al., 2009, Free Radic Biol Med. 47 (10): 1354-61.