Human PAN2 Gene cDNA Clone / ORF Clone

PAN2 cDNA Clone Datasheet

PAN2 cDNA Clone Product Information

• Gene Bank Ref.ID: BC024043
• cDNA Size: 3597
• Gene Synonym: FLJ39360, KIAA0710, USP52,PAN2
• Source: Human

PAN2 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 900 T/A not causing the amino acid variation.

PAN2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PAN2 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PAN2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAACTTTG AGGGTCTGGA CCCTGGACTG GCAGAATATG CCCCAGCCAT GCATTCTGCC
61       CTGGACCCTG TCTTGGATGC CCACCTGAAC CCAAATCTGC TACAGAATGT GGAGCTGGAC
121      CCAGAGGGAG TGGCCTTGGA GGCTCTTCCC GTCCAGGAAT CAGTGCACAT AATGGAAGGT
181      GTCTACTCTG AATTGCACAG CGTGGTGGCT GAAGTGGGTG TACCTGTTTC CGTCTCCCAC
241      TTTGACTTGC ACGAGGAGAT GCTGTGGGTG GGGAGCCACG GGGGCCATGC CACTTCATTT
301      TTTGGCCCAG CCTTGGAGCG CTACTCATCC TTTCAAGTCA ATGGCAGTGA TGATATTCGG
361      CAGATCCAGA GCCTGGAGAA TGGTATCCTT TTTCTCACCA AGAACAACCT CAAGTATATG
421      GCCCGTGGGG GCCTCATTAT ATTTGATTAC CTGCTGGATG AGAATGAGGA TATGCACAGT
481      CTCCTACTGA CTGACAGCAG CACTCTACTC GTTGGTGGGC TGCAGAATCA CATACTAGAG
541      ATTGATCTTA ACACTGTCCA GGAGACTCAG AAGTATGCAG TAGAGACGCC TGGAGTCACC
601      ATCATGAGAC AGACAAATCG CTTCTTCTTC TGCGGCCACA CGTCTGGCAA GGTTTCCCTG
661      AGAGACCTCC GTACTTTTAA GGTGGAACAT GAGTTTGATG CCTTCTCAGG AAGTCTGTCA
721      GACTTTGATG TGCATGGCAA CCTGCTAGCT GCCTGTGGCT TCTCCAGCCG CCTCACTGGC
781      CTGGCCTGCG ACCGTTTCCT CAAGGTGTAT GATTTGCGCA TGATGCGTGC CATCACACCA
841      CTTCAAGTAC ATGTGGATCC TGCCTTCTTG CGCTTCATTC CTACATATAC TTCTCGTCTA
901      GCTATCATCT CTCAGTCAGG GCAGTGCCAA TTCTGTGAAC CCACAGGCCT GGCCAACCCA
961      GCCGATATCT TTCATGTGAA TCCTGTGGGG CCTCTGCTAA TGACATTTGA TGTGTCAGCC
1021     AGCAAGCAGG CTCTGGCCTT TGGGGATTCT GAGGGCTGTG TGCACCTCTG GACTGATTCC
1081     CCGGAGCCTT CCTTCAACCC CTACTCCCGT GAGACTGAGT TTGCTTTGCC GTGTCTCGTG
1141     GACTCACTGC CTCCTCTGGA CTGGAGCCAG GACCTGCTGC CTCTTTCCCT CATCCCTGTC
1201     CCACTCACCA CTGACACACT TCTCTCTGAT TGGCCTGCTG CCAACTCTGC TCCAGCTCCC
1261     AGGCGAGCAC CACCCGTGGA TGCAGAGATT CTGCGCACCA TGAAGAAGGT GGGCTTCATT
1321     GGCTATGCGC CCAATCCCCG CACCAGGCTG CGCAATCAGA TACCCTACAG ACTCAAGGAG
1381     TCAGACAGTG AATTTGACAG CTTCAGCCAG GTCACTGAGT CACCAGTAGG ACGAGAAGAG
1441     GAACCACATC TCCACATGGT TTCTAAGAAA TACCGCAAGG TGACCATCAA ATATTCCAAG
1501     CTAGGGCTGG AGGACTTTGA CTTCAAACAC TACAATAAGA CCTTGTTTGC TGGATTAGAG
1561     CCCCACATTC CCAACGCCTA CTGTAACTGC ATGATCCAGG TGCTCTATTT CCTGGAGCCT
1621     GTACGCTGTC TAATTCAAAA CCACCTTTGC CAGAAGGAGT TCTGTCTGGC ATGTGAGCTG
1681     GGCTTCCTGT TTCACATGTT GGACCTCTCT CGTGGTGACC CTTGCCAGGG CAATAATTTT
1741     CTTCGGGCAT TCCGTACTAT TCCTGAGGCC TCAGCCCTCG GTCTAATCCT GGCTGACTCA
1801     GATGAGGCCT CAGGCAAGGG CAATCTGGCC AGGCTCATTC AGAGGTGGAA TCGCTTCATT
1861     CTCACTCAAC TGCATCAAGA TATGCAGGAG CTGGAAATAC CACAGGCTTA TCGAGGTGCT
1921     GGAGGCAGCA GCTTTTGCTC ATCGGGGGAC TCTGTTATTG GGCAGCTCTT CAGCTGTGAG
1981     ATGGAGAACT GCAGCCTCTG CCGCTGTGGC AGTGAGACCG TGCGAGCCTC ATCCACTCTG
2041     CTTTTCACAC TCTCCTACCC TGATGATAAA ACTGGGAAGA ACTATGACTT TGCTCAGGTG
2101     CTGAAGCGAA GCATCTGCCT GGACCAGAAT ACACAGGCCT GGTGTGACAC CTGTGAAAAG
2161     TACCAGCCCA CGATTCAGAC CCGCAACATC CGCCATCTGC CAGATATTCT TGTCATCAAT
2221     TGTGAGGTGA ACAGCTCAAA AGAGGCTGAT TTCTGGAGAA TGCAGGCTGA GGTTGCCTTC
2281     AAGATGGCAG TAAAGAAACA CGGTGGGGAA ATCTCCAAGA ACAAGGAATT TGCTTTGGCT
2341     GATTGGAAGG AACTAGGGAG TCCAGAGGGT GTGCTGGTGT GTCCCTCCAT TGAGGAGTTG
2401     AAGAACGTCT GGCTTCCTTT CTCCATTCGC ATGAAGATGA CCAAAAACAA AGGGCTGGAT
2461     GTTTGCAATT GGACTGATGG GGATGAGATG CAGTGGGGCC CAGCCAGGGC AGAGGAGGAG
2521     CATGGTGTCT ATGTGTATGA CCTGATGGCT ACTGTGGTAC ACATCCTGGA CTCACGCACA
2581     GGGGGCAGCC TGGTGGCTCA CATCAAAGTT GGAGAGACCT ACCACCAGCG CAAGGAGGGC
2641     GTTACTCACC AGCAGTGGTA TCTGTTCAAT GACTTTCTTA TTGAACCTAT TGATAAGCAT
2701     GAAGCTGTGC AGTTTGACAT GAATTGGAAA GTACCTGCAA TCCTTTATTA TGTCAAACGG
2761     AATCTCAATT CCAGATACAA CCTGAACATC AAGAACCCTA TTGAGGCAAG TGTCTTGCTG
2821     GCTGAAGCCT CGCTGGCACG GAAGCAGCGG AAAACACATA CTACCTTTAT TCCACTGATG
2881     CTGAATGAGA TGCCACAGAT TGGGGACCTG GTGGGTCTGG ATGCTGAGTT TGTCACCCTT
2941     AATGAGGAGG AAGCAGAGTT ACGCAGTGAT GGTACCAAGT CTACCATTAA ACCAAGCCAG
3001     ATGTCAGTAG CCAGGATTAC CTGTGTTCGG GGCCAGGGAC CCAATGAGGG TATCCCCTTC
3061     ATTGATGACT ACATCTCTAC CCAGGAGCAG GTGGTGGATT ACTTGACTCA ATACTCGGGT
3121     ATAAAGCCTG GTGACCTCGA TGCCAAAATT TCCTCCAAGC ACCTAACAAC TCTCAAGTCT
3181     ACCTACTTAA AGCTTCGTTT TCTCATTGAC ATTGGAGTCA AGTTTGTGGG TCATGGCCTG
3241     CAGAAGGACT TCCGGGTCAT CAACCTGATG GTGCCCAAGG ACCAAGTCCT TGACACTGTC
3301     TACCTGTTCC ATATGCCCCG AAAACGAATG ATTTCCCTGC GATTCCTTGC TTGGTACTTT
3361     CTGGACCTGA AGATTCAAGG GGAAACCCAT GACAGTATTG AGGATGCCCG CACAGCCCTT
3421     CAGCTGTACC GAAAGTATCT GGAGCTAAGC AAAAATGGCA CTGAGCCTGA GTCTTTCCAC
3481     AAGGTGCTCA AGGGTCTTTA TGAGAAGGGC AGAAAGATGG ACTGGAAGGT GCCTGAGCCT
3541     GAGGGCCAAA CAAGTCCCAA GAATGCAGCT GTCTTCTCCT CAGTGCTGGC GCTCTGA

 

Quality Control:cDNA sequencing results is available upon request.