Human PAM Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13624-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PAM cDNA Clone Datasheet

PAM cDNA Clone Product Information

Gene Bank Ref.ID:	BC018127
cDNA Size:	2601
Gene Synonym:	PAL;PHM;PAM
Source:	Human

PAM cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens peptidylglycine alpha-amidating monooxygenase DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

PAM cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PAM cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PAM cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGGCC GCGTCCCTAG CCTGCTAGTT CTCCTTGTTT TTCCAAGCAG CTGTTTGGCT
61       TTCCGAAGCC CACTTTCTGT CTTTAAGAGG TTTAAAGAAA CTACCAGACC ATTTTCCAAT
121      GAATGTCTTG GTACCACCAG ACCCGTAGTT CCTATTGATT CATCAGATTT TGCATTGGAT
181      ATTCGCATGC CTGGGGTTAC ACCTAAACAG TCCGATACAT ACTTCTGCAT GTCTATGCGA
241      ATACCAGTGG ATGAGGAAGC CTTCGTGATT GACTTCAAGC CTCGAGCCAG CATGGATACT
301      GTCCATCACA TGTTACTTTT TGGATGCAAT ATGCCTTCAT CCACTGGAAG TTACTGGTTT
361      TGTGATGAAG GAACCTGTAC AGATAAAGCC AATATTCTGT ATGCCTGGGC GAGAAATGCT
421      CCCCCTACCC GGCTCCCCAA AGGTGTTGGA TTCAGAGTTG GAGGAGAGAC TGGAAGTAAA
481      TACTTTGTAC TACAGGTACA CTATGGGGAT ATTAGTGCTT TTAGAGATAA TAACAAGGAC
541      TGTTCTGGTG TGTCCTTACA CCTCACACGT CTGCCACAGC CTTTAATTGC TGGCATGTAC
601      CTTATGATGT CTGTTGACAC TGTTATCCCA GCAGGAGAAA AAGTGGTGAA TTCTGACATT
661      TCATGCCATT ATAAAAATTA TCCAATGCAT GTCTTTGCCT ATAGAGTTCA CACTCACCAT
721      TTAGGTAAGG TAGTAAGTGG ATACAGAGTA AGAAATGGAC AGTGGACACT GATTGGACGG
781      CAGAGCCCTC AGCTGCCACA GGCTTTCTAC CCTGTGGGGC ATCCAGTTGA TGTAAGTTTT
841      GGTGACCTAC TGGCTGCAAG ATGTGTATTC ACTGGTGAAG GAAGGACAGA AGCCACACAC
901      ATTGGTGGCA CGTCTAGTGA TGAAATGTGC AACTTATACA TTATGTATTA CATGGAAGCC
961      AAGCATGCAG TTTCTTTCAT GACCTGTACC CAGAATGTAG CTCCAGATAT GTTCAGAACC
1021     ATACCACCAG AGGCCAACAT TCCAATTCCC GTGAAGTCTG ATATGGTTAT GATGCATGAA
1081     CATCATAAAG AAACAGAATA TAAAGATAAG ATTCCTTTAC TACAGCAGCC AAAACGAGAA
1141     GAAGAAGAAG TGTTAGACCA GGATTTCCAC ATGGAAGAGG CACTGGATTG GCCTGGAGTA
1201     TACTTGTTAC CAGGCCAGGT TTCTGGGGTG GCTCTAGACC CTAAGAATAA CCTGGTGATT
1261     TTCCACAGAG GTGACCATGT CTGGGATGGA AACTCGTTTG ACAGCAAGTT TGTTTACCAG
1321     CAAATAGGAC TCGGACCAAT TGAAGAAGAC ACTATTCTTG TCATAGATCC AAATAATGCT
1381     GCAGTACTCC AGTCCAGTGG AAAAAATCTG TTTTACTTGC CACATGGCTT GAGTATAGAT
1441     AAAGATGGGA ATTATTGGGT CACAGACGTG GCTCTCCATC AGGTGTTCAA ACTGGATCCA
1501     AACAATAAAG AAGGCCCTGT ATTAATCCTG GGAAGGAGCA TGCAACCAGG CAGTGACCAG
1561     AATCACTTCT GTCAACCCAC TGATGTGGCT GTGGATCCAG GCACTGGAGC CATTTATGTA
1621     TCAGATGGTT ACTGCAACAG CAGGATTGTG CAGTTTTCAC CAAGTGGAAA GTTCATCACA
1681     CAGTGGGGAG AAGAGTCTTC AGGGAGCAGT CCTCTGCCAG GCCAGTTCAC TGTTCCTCAC
1741     AGCTTGGCTC TTGTGCCTCT TTTGGGCCAA TTATGTGTGG CAGACCGGGA AAATGGTCGG
1801     ATCCAGTGTT TTAAAACTGA CACCAAAGAA TTTGTGAGAG AGATTAAGCA TTCATCATTT
1861     GGAAGAAATG TATTTGCAAT TTCATATATA CCAGGCTTGC TCTTTGCAGT GAATGGGAAG
1921     CCTCATTTTG GGGACCAAGA ACCTGTACAA GGATTTGTGA TGAACTTTTC CAATGGGGAA
1981     ATTATCGACA TCTTCAAGCC AGTGCGCAAG CACTTTGATA TGCCTCATGA TATTGTTGCA
2041     TCTGAAGATG GGACTGTGTA CATTGGAGAT GCTCATACCA ACACCGTGTG GAAGTTCACC
2101     TTGACTGAGA AATTGGAACA TCGATCAGTT AAAAAGGCTG GCATTGAGGT CCAGGAAATC
2161     AAAGAAGCCG AGGCAGTTGT TGAAACCAAA ATGGAGAACA AACCCACCTC CTCAGAATTG
2221     CAGAAGATGC AAGAGAAACA GAAACTGATC AAAGAGCCAG GCTCGGGAGT GCCTGTTGTT
2281     CTCATTACAA CCCTTCTGGT TATTCCGGTG GTTGTCCTGC TGGCCATTGC CATATTTATT
2341     CGGTGGAAAA AATCAAGGGC CTTTGGAGAT TCTGAACACA AACTCGAGAC GAGTTCAGGA
2401     AGAGTACTGG GAAGATTTAG AGGAAAGGGA AGTGGAGGCT TAAACCTTGG TAATTTCTTT
2461     GCAAGCCGTA AGGGCTACAG TCGAAAAGGG TTTGACCGGC TTAGCACTGA GGGCAGTGAC
2521     CAAGAGAAAG AGGATGATGG AAGTGAATCA GAAGAGGAGT ATTCAGCACC TCTGCCTGCG
2581     CTCGCACCTT CCTCCTCCTG A

Quality Control:cDNA sequencing results is available upon request.