Mouse PAK7 Gene cDNA Clone / ORF Clone

PAK7 cDNA Clone Datasheet

PAK7 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_172858.2
• cDNA Size: 2160
• Gene Synonym: Pak5; MGC117585; 6430627N20; 2900083L08Rik; Pak7
• Source: Mouse

PAK7 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus p21 protein (Cdc42/Rac)-activated kinase 7 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

PAK7 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PAK7 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PAK7 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTTGGGA AGAAAAAGAA AAAGATCGAA ATATCTGGCC CATCCAACTT TGAACACAGG
61       GTTCATACTG GATTTGATCC ACAAGAGCAG AAGTTTACTG GCCTTCCCCA GCAGTGGCAC
121      AGCCTGTTAG CAGACACAGC CAACAGGCCC AAGCCCATGG TGGACCCATC ATGCATCACA
181      CCCATACAGC TGGCTCCCAT GAAGACAATC GTCAGAGGAA ATAAATCCTG CAAGGAAACC
241      TCTATCAATG GTCTGCTAGA GGATTTTGAC AACATCTCCG TGACTCGCTC CAACTCTCTA
301      AGGAAAGAAA GCCCACCCAC CCCAGATCAG GGAGCAGCTA GCCGCATTCA AGGCCACTCG
361      GAAGAGAACG GCTTCATCAC TTTCTCACAA TATTCCAGTG AATCCGATAC GACTGCGGAC
421      TACACAACTG AAAAGTACAG AGACAGGAGT CTCTATGGAG ATGACCTGGA TCTGTACTAT
481      AAAAGCAGCC ATGCAGCCAA GCAAAATGGG CATGCCATGA AGATGAAACA TGGAGACGCT
541      TACTACCCTG AGATGAAGTC TTTGAAAACC GACCTGGCCG GATTCCCTGT CGACTATCAC
601      ACCCACTTGG ACTCTCTGAG AAAATCAAGT GAATATGGTG ACCTTAGGTG GGATTATCAG
661      AGAGCCTCTA GTAGCTCCCC TCTGGACTAC TCATTCCAGC TCACGCCTTC TAGAACTGCA
721      GGGACCAGCA GGTGCTCCAA GGAGAGTCTG GCATACAGTG AAAGTGATTG GGGACCCAGC
781      CTGGATGACT ATGACAGGAG GCCAAAATCA TCATACCTGC ATCAGACGAG CCCTCAGCCA
841      GCCATGCGCC AGAGATCCAA GTCCGGCTCA GGGCTTCAGG AACCCATGAT GCCATTTGGA
901      GCAAGTGCAT TTAAAACTCA TCCTCAAGGA CACTCGTACA ACTCCTACAC CTACCCTCGA
961      TTGTCCGAGC CCACAATGTG CATTCCAAAG GTGGATTACG ATCGAGCACA GATGGTCTTC
1021     AGTCCTCCAC TGTCAGGGTC CGACACTTAC CCCAGAGGCC CCACCAAACT ACCTCAAAGT
1081     CAAAGCAAAG CAGGCTACTC TTCAGGCAGC CACCAGTACC CTTCTGGGTA CCACAAAGCA
1141     TCTCTATACC ACCATCCATC CCTGCAAACC AGTTCTCAGT ACATCTCCAC CGCTTCTTAC
1201     TTAAGCTCTC TCAGTATCTC CTCGAGCACC TACCCTCCAC CTAGCTGGGG CTCCTCCTCA
1261     GACCAGCAGC CCTCAAGGGT ATCCCATGAA CAATTCCGAG CTGCCCTGCA ACTGGTGGTC
1321     AGCCCAGGAG ACCCCAGGGA ATATTTGGAT AACTTTATTA AAATCGGAGA AGGGTCGACA
1381     GGCATCGTGT GCATTGCAAC AGAGAAACAC ACAGGCAAGC AAGTGGCAGT GAAGAAAATG
1441     GACCTCCGAA AGCAGCAGAG ACGGGAACTC CTTTTTAATG AGGTCGTGAT AATGCGTGAT
1501     TACCACCATG ACAACGTAGT TGACATGTAC AACAGCTACC TTGTTGGAGA TGAGCTCTGG
1561     GTGGTCATGG AGTTTCTAGA AGGTGGTGCC TTGACAGACA TTGTCACTCA TACCAGAATG
1621     AATGAAGAGC AGATAGCTAC TGTCTGCCTG TCAGTTCTGA AAGCTCTGTC CTACCTTCAT
1681     AACCAAGGAG TGATTCACAG GGACATAAAG AGTGACTCCA TTCTTCTGAC AAGCGATGGC
1741     CGGATAAAGT TATCTGACTT TGGTTTCTGT GCTCAAGTTT CCAAAGAGGT GCCAAAGAGG
1801     AAGTCACTGG TGGGTACCCC ATACTGGATG GCACCTGAGG TGATTTCCAG GCTACCTTAT
1861     GGGACAGAGG TGGACATCTG GTCCCTCGGG ATAATGGTGA TAGAGATGAT TGATGGGGAG
1921     CCCCCCTATT TCAATGAGCC TCCTCTGCAG GCCATGAGGA GGATCCGGGA CAGTTTACCT
1981     CCAAGAGTGA AGGACCTACA CAAGGTTTCT TCCATGCTCC GAGGATTCCT AGATCTTATG
2041     TTGGTGAGGG AGCCCTCTCA AAGAGCCACA GCTCAAGAAC TCCTTGGACA TCCATTCTTA
2101     AAATTGGCAG GTCCACCATC TTGCATTGTT CCTCTCATGA GACAATACAG ACATCACTGA

 

Quality Control:cDNA sequencing results is available upon request.