PAI-1 / SerpinE1 Antibody

PAI-1 / SerpinE1 Antibody Datasheet

PAI-1 / SerpinE1 Antibody Product Information

• Immunogen: Recombinant Human PAI-1 / SerpinE1 Protein (Catalog # 10296-H08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 004
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human PAI-1 / SerpinE1 (rh PAI-1 / SerpinE1; Catalog # 10296-H08H; NP_000593.1; Met 1 - Pro 402).

PAI-1 / SerpinE1 Antibody Usage Guide

• Specificity: Human PAI-1 / SerpinE1
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human SERPINE1 in WB. Using a DAB detection system, the detection limit for Human SERPINE1 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SERPINE1. The detection limit for Human SERPINE1 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

PAI-1 / SerpinE1 Antibody Background

Serpins are the largest and most diverse family of serine protease inhibitors which are involved in a number of fundamental biological processes such as blood coagulation, complement activation, fibrinolysis, angiogenesis, inflammation and tumor suppression and are expressed in a cell-specific manner. Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. The acronym serpin was originally coined because many serpins inhibit chymotrypsin-like serine proteases (serine protease inhibitors). Over 1000 serpins have been identified. Plasminogen activator inhibitor 1, also known as PAI-1, Endothelial plasminogen activator inhibitor, SERPINE1 and PLANH1, is a secreted protein which belongs to theserpin family. SERPINE1 acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Defects in PAI-1/SerpinE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency) which is characterized by abnormal bleeding due to PAI-1/SerpinE1 defect in the plasma. High concentrations of PAI-1/SerpinE1 have been associated with thrombophilia which is an autosomal dominant disorder in which affected individuals are prone to develop serious spontaneous thrombosis.

References

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