Anti-PAI-1 Antibody

Cat: 10296-RP02
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Anti-PAI-1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-PAI-1 Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human PAI-1
Immunogen
Recombinant Human SerpinE1 protein (Catalog#10296-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human SerpinE1 (rh SerpinE1; Catalog#10296-H08H; NP_000593.1; Met 1-Pro 402). SerpinE1 specific IgG was purified by human SerpinE1 affinity chromatography .
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-PAI-1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-PAI-1 Antibody Alternative Names
Anti-PAI Antibody;Anti-PAI-1 Antibody;Anti-PAI1 Antibody;Anti-PLANH1 Antibody;Anti-SERPINE1 Antibody
PAI-1 Background Information

Plasminogen activator inhibitor 1, also known as PAI-1, Endothelial plasminogen activator inhibitor, SerpinE1 and PLANH1, is a secreted glycoprotein which belongs to the serpin family. SerpinE1 is the primary physiological inhibitor of the two plasminogen activators urokinase (uPA) and tissue plasminogen activator (tPA). Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Defects in SerpinE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency) which is characterized by abnormal bleeding due to SerpinE1 defect in the plasma. High concentrations of SerpinE1 have been associated with thrombophilia which is an autosomal dominant disorder in which affected individuals are prone to develop serious spontaneous thrombosis. Studies of PAI-1 have contributed significantly to the elucidation of the protease inhibitory mechanism of serpins, which is based on a metastable native state becoming stabilised by insertion of the RCL into the central beta-sheet A and formation of covalent complexes with target proteases. Greater expression of PAI-1 has been associated with increased survival of cells and resistance to apoptosis. PAI-1 appears to influence apoptosis by decreasing cell adhesion (anoikis) as well as its effect on intracellular signaling. PAI-1, in its active state, also binds to the extracellular protein vitronectin. When in complex with its target proteases, it binds with high affinity to endocytosis receptors of the low density receptor family. The mechanisms of PAI-1 overexpression during obesity are complex, and it is conceivable that several inducers are involved at the same time at several sites of synthesis. PAI-1 is also implicated in adipose tissue development. It suggests that PAI-1 inhibitors serve in the control of atherothrombosis.

Full Name
serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
References
  • Alessi MC, et al. (2006) PAI-1 and the metabolic syndrome: links, causes, and consequences. Arterioscler Thromb Vasc Biol. 26(10): 2200-7.
  • Schneider DJ, et al. (2008) The effect of plasminogen activator inhibitor type 1 on apoptosis. Thromb Haemost. 100(6): 1037-40.
  • Dupont DM, et al. (2009) Biochemical properties of plasminogen activator inhibitor-1. Front Biosci. 14: 1337-61.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-PAI-1 Antibody Rabbit Polyclonal ELISA 80442-RP01
    Anti-PAI-1 Antibody Rabbit Polyclonal ELISA 80442-T16
    Anti-PAI-1 Antibody Rabbit Monoclonal ELISA 80442-R001
    Anti-PAI-1 Antibody Mouse Monoclonal ELISA(Cap) 10296-MM01
    Anti-PAI-1 Antibody Rabbit Polyclonal WB,ELISA 10296-RP01
    Anti-PAI-1 Antibody Rabbit Monoclonal ELISA,ELISA(Det) 10296-R004
    Anti-PAI-1 Antibody Rabbit Polyclonal ELISA 10296-T16
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