P63 / TP63 / Tumor protein p63 Protein (GST Tag)

Tumor protein p63 Protein Datasheet

P63 / TP63 / Tumor protein p63 Protein Product Information

• Synonym: AIS, B(p51A), B(p51B), EEC3, KET, LMS, NBP, OFC8, RHS, SHFM4, TP53CP, TP53L, TP73L, p40, p51, p53CP, p63, p73H, p73L
• Protein Construction: A DNA sequence encoding the human TP63 isoform 1(Q9H3D4-1) (Met 1-Glu 680) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

P63 / TP63 / Tumor protein p63 Protein QC Testing

• Purity: >90 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human TP63/GST chimera consists of 917 amino acids and has a calculated molecular mass of 105 KDa. It migrates as an approximately 125 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 0.3mM DTT, 20%glycerol
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: P63 / TP63 / Tumor protein p63 protein

P63 / TP63 / Tumor protein p63 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

P63 / TP63 / Tumor protein p63 Protein Description

Tumor protein p63 is a protein also known as transformation-related protein 63, TP63, and p63. Tumor protein p63 / p63 is a member of the p53 family of transcription factors whose members P53, p63, and p73 have similar features in their gene structures and functions. An animal model, p63-/- mice has been useful in difining the role p63/TP63 plays in the development and maintenance of stratified epithelial tissues. This p63/TP63 encoding protein p63/TP63 has a dramatic impact on replenishment of cutaneous epithelial stem cells and on ovarian germ cell survival. Although these two fundamental roles of p63/TP63 attest to its powerful place in development, its other functions, specifically the apparent capacity of p63/TP63, is to supervise the emergence of new cell populations in the breast, prostate, cervix, and upper reproductive tract. P63-/- mice have several development defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium. Mutations in this protein are associated with ectodermal dysplasia, and cleft lip / palate syndrome 3, ADULT syndrome (acro-dermato-ungual-lacrimal-tooth), limb-mammary syndrome, et al.

References

1. Crum CP. et al., 2010, Annu Rev Pathol. 5: 349-71.
2. Tan M. et al., 2001, Carcinogenesis. 22 (2): 295-300.
3. Shiran MS. et al., 2007, Med J Malaysia. 62 (1): 36-9.