P4HB Matched ELISA Antibody Pair Set,Human

Cat: SEK10827
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P4HB Matched ELISA Antibody Pair Set,Human General Information
Product name
P4HB Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human P4HB
Assay range
46.88-3000
Materials provided
1. Capture Antibody 1 mg/mL of mouse anti-Human P4HB monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10827-MM12)
2. Detection Antibody 0.2 mg/mL of mouse anti-Human P4HB monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10827-MM08)
3. Standard Each vial contains 85 ng of recombinant Human P4HB. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3000 pg/mL is recommended.
Product overview
This P4HB Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human P4HB . It contains Human P4HB capture antibody, Human P4HB detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human P4HB protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
P4HB Matched ELISA Antibody Pair Set,Human Images
Human P4HB ELISA Pair Set Standard Curve 7104
P4HB Matched ELISA Antibody Pair Set,Human Alternative Names
DSI Matched ELISA Antibody Pair Set, Human;ERBA2L Matched ELISA Antibody Pair Set, Human;GIT Matched ELISA Antibody Pair Set, Human;P4Hbeta Matched ELISA Antibody Pair Set, Human;PDI Matched ELISA Antibody Pair Set, Human;PDIA1 Matched ELISA Antibody Pair Set, Human;PHDB Matched ELISA Antibody Pair Set, Human;PO4DB Matched ELISA Antibody Pair Set, Human;PO4HB Matched ELISA Antibody Pair Set, Human;PROHB Matched ELISA Antibody Pair Set, Human
P4HB Background Information

Protein disulfide-isomerase, also known as Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55 and P4HB, is a peripheral membrane protein which belongs to the protein disulfide isomerase family. P4HB is highly abundant. In some cell types, it seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. P4HB localizes near CD4-enriched regions on lymphoid cell surfaces. It is identified by mass spectrometry in melanosome fractions from stage I to stage IV. P4HB reduces and may activate fusogenic properties of HIV-1 gp120 surface protein, thereby enabling HIV-1 entry into the cell. P4HB catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. P4HB may therefore cause structural modifications of exofacial proteins. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). P4HB may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.

Full Name
prolyl 4-hydroxylase, beta polypeptide
References
  • Kivirikko KI, et al., 1989, FASEB J., 3 (5): 1609-17.
  • Pihlajaniemi T, et al.,1991, J Hepatol., 13, Suppl 3: S2
  • Fenouillet E., et al., 2001, J. Infect. Dis. 183:744-752.
  • Gevaert K., et al., 2003, Nat. Biotechnol. 21:566-569.
  • Barbouche R., et al., 2003, J. Biol. Chem. 278:3131-3136.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    P4HB ELISA Kit, Human Lysate KIT10827
    info info
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