P4HB Antibody

P4HB Antibody Datasheet

P4HB Antibody Product Information

• Immunogen: Recombinant Mouse P4HB protein (Catalog#50638-M08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse P4HB (rM P4HB; Catalog#50638-M08H; NP_035162.1; Met 1-Lys 506). Total IgG was purified by Protein A affinity chromatography.

P4HB Antibody Usage Guide

• Specificity: Mouse P4HB
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse P4HB in WB.
• Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Mouse P4HB. The detection limit for Mouse P4HB is approximately 0.0049 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

P4HB Antibody Background

Prolyl 4-hydroxylase belongs to the group of 2-oxoglutarate coupled dioxygenases, and catalyzes the formation of 4-hydroxyproline in collagens by the hydroxylation of proline residues in peptide linkages. The reaction product, the genetically uncoded amino acid 4-hydroxyproline, plays a crucial role in collagens, as the hydroxyl groups of these amino acid residues are essential for the folding of the newly synthesized procollagen polypeptide chains into triple-helical molecules. The active enzyme is a tetramer (α2β2), and the β subunit (P4HB) alnoe has been found to be identical to the enzyme protein disulfide isomerase (PDI). P4HB is multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. Inside the cell, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins at high concentrations, while facilitates aggregation (anti-chaperone activity) at low concentrations. In addition to a subunit of polyl 4-hydroxylase, P4HB also acts as a structure subunit of microsomal triacylglycerol transfer protein MTTP.

References

1. Kivirikko KI, et al., 1989, FASEB J., 3 (5): 1609-17.
2. Pihlajaniemi T, et al., 1991, J Hepatol., 13, Suppl 3: S2-7.