Anti-P4HB Antibody
Cat: 10827-R016
Anti-P4HB Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-P4HB Antibody
Validated applications
WB,ELISA,ICC/IF,IF,IP
Species reactivity
Reacts with: Human
Specificity
Human P4HB
Immunogen
Recombinant Human P4HB protein (Catalog#10827-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human P4HB / ERBA2L (rh P4HB; Catalog#10827-H08H; NP_000909.2; Met 1-Lys 505).
Source
Monoclonal Rabbit IgG Clone #16
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Anti-P4HB Antibody (Rabbit Monoclonal antibody) Validated Applications
| Application | Dilution |
|---|---|
| WB | 1:500-1:2000 |
| ELISA | 1:5000-1:10000 |
| ICC-IF | 1:20-1:100 |
| IP | 1-4 μL/mg of lysate |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Anti-P4HB Antibody (Rabbit Monoclonal antibody) Images
Immunofluorescence staining of Human P4HB in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human P4HB monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to endoplasmic reticulum.
P4HB was immunoprecipitated using:
Lane A:0.5 mg MCF-7 Whole Cell Lysate
2 µL anti-P4HB rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:Anti-P4HB rabbit monoclonal antibody,at 1:200 dilution
Secondary antibody:Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution
Developed using the DAB staining technique.Performed under reducing conditions.
Predicted band size: 57 kDaObserved band size: 57 kDa
Anti-P4HB rabbit monoclonal antibody at 1:100 dilution
Lane A: MCF7 Whole Cell Lysate
Lane B: HepG2 Whole Cell Lysate
Lysates/proteins at 30 μg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size:57 kDa
Observed band size:57 kDa
(We are unsure as to the identity of these extra bands.)
Anti-P4HB Antibody: Alternative Names
Anti-DSI Antibody; Anti-ERBA2L Antibody; Anti-GIT Antibody; Anti-P4Hbeta Antibody; Anti-PDI Antibody; Anti-PDIA1 Antibody; Anti-PHDB Antibody; Anti-PO4DB Antibody; Anti-PO4HB Antibody; Anti-PROHB Antibody
P4HB Background Information
Protein disulfide-isomerase, also known as Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55 and P4HB, is a peripheral membrane protein which belongs to the protein disulfide isomerase family. P4HB is highly abundant. In some cell types, it seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. P4HB localizes near CD4-enriched regions on lymphoid cell surfaces. It is identified by mass spectrometry in melanosome fractions from stage I to stage IV. P4HB reduces and may activate fusogenic properties of HIV-1 gp12 surface protein, thereby enabling HIV-1 entry into the cell. P4HB catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. P4HB may therefore cause structural modifications of exofacial proteins. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). P4HB may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
Full Name
prolyl 4-hydroxylase, beta polypeptide
Research Areas
- Cell Biology>>Apoptosis>>Oxidative Stress>>Additional Reduction/Oxidation (Redox) Enzymes
- Neuroscience>>Cell Adhesion Proteins>>ECM Proteins>>Collagen
- Developmental Biology>>Metabolism>>Pathways and Processes>>Metabolic signaling pathways>>Lipid and lipoprotein metabolism>>Lipid metabolism
- Developmental Biology>>Metabolism>>Types of disease>>Metabolism in Cancer
- Cancer>>Cancer Metabolism>>Metabolic signaling>>Metabolism of lipids and lipoprotein
- Cancer>>Signal Transduction>>Protein Trafficking>>Chaperones>>Other Chaperones
References
- Kivirikko KI, et al., 1989, FASEB J., 3 (5): 1609-17.
- Pihlajaniemi T, et al.,1991, J Hepatol., 13, Suppl 3: S2
- Fenouillet E., et al., 2001, J. Infect. Dis. 183:744-752.
- Gevaert K., et al., 2003, Nat. Biotechnol. 21:566-569.
- Barbouche R., et al., 2003, J. Biol. Chem. 278:3131-3136.
