Application | Dilution |
---|---|
WB | 1:500-1:2000 |
ELISA | 1:5000-1:10000 |
ICC-IF | 1:20-1:100 |
IP | 1-4 μL/mg of lysate |
P4HB was immunoprecipitated using:
Lane A:0.5 mg MCF-7 Whole Cell Lysate
2 µL anti-P4HB rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:Anti-P4HB rabbit monoclonal antibody,at 1:200 dilution
Secondary antibody:Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution
Developed using the DAB staining technique.Performed under reducing conditions.
Predicted band size: 57 kDaObserved band size: 57 kDa
Anti-P4HB rabbit monoclonal antibody at 1:100 dilution
Lane A: MCF7 Whole Cell Lysate
Lane B: HepG2 Whole Cell Lysate
Lysates/proteins at 30 μg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size:57 kDa
Observed band size:57 kDa
(We are unsure as to the identity of these extra bands.)