Anti-P4HB Antibody

Cat: 10827-MM08
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Anti-P4HB Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-P4HB Antibody
Validated applications
ELISA,ELISA(Det),IHC-P
Species reactivity
Reacts with: Human
Specificity
Human P4HB
Immunogen
Recombinant Human P4HB protein (Catalog#10827-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human P4HB / ERBA2L (rh P4HB; Catalog#10827-H08H; NP_000909.2; Met 1-Lys 505). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #2F6G12H2
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-P4HB Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
IHC-P 1:20-1:80  
ELISA(Det) 1:1000-1:10000 This antibody will detect Human P4HB in ELISA pair set (Catalog: # SEK10827). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10827-MM12).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-P4HB Antibody (Mouse Monoclonal antibody) Images
Human P4HB Immunohistochemistry(IHC) 3828
Immunochemical staining of human P4HB in human small intestine with mouse monoclonal antibody (1:30, formalin-fixed paraffin embedded sections).
Human P4HB Immunohistochemistry(IHC) 3830
Immunochemical staining of human P4HB in human liver with mouse monoclonal antibody (1:30, formalin-fixed paraffin embedded sections).
Human P4HB Immunohistochemistry(IHC) 3832
Immunochemical staining of human P4HB in human brain with mouse monoclonal antibody (1:30, formalin-fixed paraffin embedded sections).
Anti-P4HB Antibody Alternative Names
Anti-DSI Antibody;Anti-ERBA2L Antibody;Anti-GIT Antibody;Anti-P4Hbeta Antibody;Anti-PDI Antibody;Anti-PDIA1 Antibody;Anti-PHDB Antibody;Anti-PO4DB Antibody;Anti-PO4HB Antibody;Anti-PROHB Antibody
P4HB Background Information

Protein disulfide-isomerase, also known as Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55 and P4HB, is a peripheral membrane protein which belongs to the protein disulfide isomerase family. P4HB is highly abundant. In some cell types, it seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. P4HB localizes near CD4-enriched regions on lymphoid cell surfaces. It is identified by mass spectrometry in melanosome fractions from stage I to stage IV. P4HB reduces and may activate fusogenic properties of HIV-1 gp120 surface protein, thereby enabling HIV-1 entry into the cell. P4HB catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. P4HB may therefore cause structural modifications of exofacial proteins. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). P4HB may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.

Full Name
prolyl 4-hydroxylase, beta polypeptide
References
  • Kivirikko KI, et al., 1989, FASEB J., 3 (5): 1609-17.
  • Pihlajaniemi T, et al.,1991, J Hepatol., 13, Suppl 3: S2
  • Fenouillet E., et al., 2001, J. Infect. Dis. 183:744-752.
  • Gevaert K., et al., 2003, Nat. Biotechnol. 21:566-569.
  • Barbouche R., et al., 2003, J. Biol. Chem. 278:3131-3136.
  • Product Description Host Clonality Application Catalog# (PDF)
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    Anti-P4HB Antibody (FITC) Mouse Monoclonal FCM 10827-MM03-F
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