Human P4HA2 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13577-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

P4HA2 cDNA Clone Datasheet

P4HA2 cDNA Clone Product Information

Gene Bank Ref.ID:	BC035813
cDNA Size:	1602
Gene Synonym:	P4HA2
Source:	Human

P4HA2 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens prolyl 4-hydroxylase, alpha polypeptide II DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

P4HA2 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

P4HA2 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

P4HA2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAACTCT GGGTGTCTGC ATTGCTGATG GCCTGGTTTG GTGTCCTGAG CTGTGTGCAG
61       GCCGAATTCT TCACCTCTAT TGGGCACATG ACTGACCTGA TTTATGCAGA GAAAGAGCTG
121      GTGCAGTCTC TGAAAGAGTA CATCCTTGTG GAGGAAGCCA AGCTTTCCAA GATTAAGAGC
181      TGGGCCAACA AAATGGAAGC CTTGACTAGC AAGTCAGCTG CTGATGCTGA GGGCTACCTG
241      GCTCACCCTG TGAATGCCTA CAAACTGGTG AAGCGGCTAA ACACAGACTG GCCTGCGCTG
301      GAGGACCTTG TCCTGCAGGA CTCAGCTGCA GGTTTTATCG CCAACCTCTC TGTGCAGCGG
361      CAGTTCTTCC CCACTGATGA GGACGAGATA GGAGCTGCCA AAGCCCTGAT GAGACTTCAG
421      GACACATACA GGCTGGACCC AGGCACAATT TCCAGAGGGG AACTTCCAGG AACCAAGTAC
481      CAGGCAATGC TGAGTGTGGA TGACTGCTTT GGGATGGGCC GCTCGGCCTA CAATGAAGGG
541      GACTATTATC ATACGGTGTT GTGGATGGAG CAGGTGCTAA AGCAGCTTGA TGCCGGGGAG
601      GAGGCCACCA CAACCAAGTC ACAGGTGCTG GACTACCTCA GCTATGCTGT CTTCCAGTTG
661      GGTGATCTGC ACCGTGCCCT GGAGCTCACC CGCCGCCTGC TCTCCCTTGA CCCAAGCCAC
721      GAACGAGCTG GAGGGAATCT GCGGTACTTT GAGCAGTTAT TGGAGGAAGA GAGAGAAAAA
781      ACGTTAACAA ATCAGACAGA AGCTGAGCTA GCAACCCCAG AAGGCATCTA TGAGAGGCCT
841      GTGGACTACC TGCCTGAGAG GGATGTTTAC GAGAGCCTCT GTCGTGGGGA GGGTGTCAAA
901      CTGACACCCC GTAGACAGAA GAGGCTTTTC TGTAGGTACC ACCATGGCAA CAGGGCCCCA
961      CAGCTGCTCA TTGCCCCCTT CAAAGAGGAG GACGAGTGGG ACAGCCCGCA CATCGTCAGG
1021     TACTACGATG TCATGTCTGA TGAGGAAATC GAGAGGATCA AGGAGATCGC AAAACCTAAA
1081     CTTGCACGAG CCACCGTTCG TGATCCCAAG ACAGGAGTCC TCACTGTCGC CAGCTACCGG
1141     GTTTCCAAAA GCTCCTGGCT AGAGGAAGAT GATGACCCTG TTGTGGCCCG AGTAAATCGT
1201     CGGATGCAGC ATATCACAGG GTTAACAGTA AAGACTGCAG AATTGTTACA GGTTGCAAAT
1261     TATGGAGTGG GAGGACAGTA TGAACCGCAC TTCGACTTCT CTAGGCGACC TTTTGACAGC
1321     GGCCTCAAAA CAGAGGGGAA TAGGTTAGCG ACGTTTCTTA ACTACATGAG TGATGTAGAA
1381     GCTGGTGGTG CCACCGTCTT CCCTGATCTG GGGGCTGCAA TTTGGCCTAA GAAGGGTACA
1441     GCTGTGTTCT GGTACAACCT CTTGCGGAGC GGGGAAGGTG ACTACCGAAC AAGACATGCT
1501     GCCTGCCCTG TGCTTGTGGG CTGCAAGTGG GTCTCCAATA AGTGGTTCCA TGAACGAGGA
1561     CAGGAGTTCT TGAGACCTTG TGGATCAACA GAAGTTGACT GA

Quality Control:cDNA sequencing results is available upon request.