- EGFR Signaling Pathway
- TGF-beta Signaling
- Canonical Wnt Signaling
- non-Canonical Wnt Signaling
- Notch Signaling
- p53 Pathway
- NF-kB Pathway
- Cytokine Signaling
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Osteopontin / SPP1 / ETA-1 Antibody Datasheet
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Osteopontin / SPP1 / ETA-1 Antibody Product Information
Recombinant Human Osteopontin / SPP1 / ETA-1 protein (Catalog#10352-H08H)
|Antibody Type :||Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )|
Clone ID :
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.|
This antibody was obtained from a rabbit immunized with purified, recombinant Human Osteopontin / SPP1 / ETA-1 (rh Osteopontin / SPP1 / ETA-1; Catalog#10352-H08H; NP_001035147.1; Met 1-Asn 314).
Osteopontin / SPP1 / ETA-1 Antibody Usage Guide
Human Osteopontin / SPP1 / ETA-1
|Western blot :||
WB: 5-10 μg/mL
|Direct ELISA :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SPP1.|
|Flow Cytometry :||
Flow cytometric analysis of purified anti-human Spp1 on U937 cells. U937 cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Rabbit anti-Spp1 (Bold line hisgram, 1 μg/test), To demonstrate specificity of staining the binding of anti-human Spp1 (10352-R001) was blocked by the preincubation of the purified antibody with molar excess of recombinant human Spp1 (5 µg, Cat. No. 10352-H08H) for 1 hour (dashed line hisgram), then stained with a FITC-conjugated second step antibody.Flow cytometry was performed on a BD FACSCalibur flow cytometry system
Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Osteopontin / SPP1 / ETA-1 Antibody Related Products & Topics
Osteopontin / SPP1 / ETA-1 Antibody Related Areas:
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|Osteopontin/SPP1/ETA-1||Human||Osteopontin/SPP1/ETA-1 Protein, Recombinant||10352-H08H|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|WB, ELISA||Rabbit Polyclonal Antibody||10352-RP01|
|WB, ELISA||Osteopontin/SPP1/ETA-1 Antibody, Rabbit PAb (Antigen Affinity Purified)||10352-RP02|
|WB, ELISA, FCM||Osteopontin / SPP1 / ETA-1 Antibody||10352-R001|
|WB, ELISA||Osteopontin / SPP1 / ETA-1 Antibody||10352-R014|
Osteopontin / SPP1 / ETA-1 Antibody Background
- Rangaswami H, et al., 2006, Trends Cell Biol.16 (2): 79-87.
- Banerjee A, et al., 2006, J. Pathol.208 (4): 473-85.
- Burdo TH, et al., 2007,J. Leukoc. Biol.81 (6): 1504-11.
- Wang KX, et al., 2008,Cytokine Growth Factor Rev.19 (5-6): 333-45.
- Nagasaka A, et al., 2008, Eur. J. Immunol.38 (2): 489-99.