Anti-Osteopontin Antibody

Cat: 10352-R001

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Anti-Osteopontin Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-Osteopontin Antibody
Validated applications
Species reactivity
Reacts with: Human
Human Osteopontin
Recombinant Human Osteopontin / SPP1 / ETA-1 protein (Catalog#10352-H08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Human Osteopontin / SPP1 / ETA-1 (rh Osteopontin / SPP1 / ETA-1; Catalog#10352-H08H; NP_001035147.1; Met 1-Asn 314).
Monoclonal Rabbit IgG Clone #001
Protein A
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Osteopontin Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  
IHC-P 1:1000-1:4000  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Osteopontin Antibody (Rabbit Monoclonal antibody) Images
Human OPN/Osteopontin Flow Cytometry (FC) 4908
Flow cytometric analysis of purified anti-human Spp1 on U937 cells. U937 cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Rabbit anti-Spp1 (Bold line hisgram), To demonstrate specificity of staining the binding of anti-human Spp1 (10352-R001) was blocked by the preincubation of the purified antibody with molar excess of recombinant human Spp1 (5 µg, Cat. No. 10352-H08H) for 1 hour (dashed line hisgram), then stained with a FITC-conjugated second step antibody.
Human EpCAM/TROP-1/TACSTD1 Immunoprecipitation(IP) 8011
Human OPN/Osteopontin Immunohistochemistry(IHC) 16053
Immunochemical staining of human SPP1 in human kidney with rabbit monoclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
Human OPN/Osteopontin Immunohistochemistry(IHC) 16054
Immunochemical staining of human SPP1 in human hepatoma with rabbit monoclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
Anti-Osteopontin Antibody Alternative Names
Anti-BNSP Antibody;Anti-BSPI Antibody;Anti-ETA-1 Antibody;Anti-OPN Antibody
Osteopontin Background Information

Osteopontin, also known as Secreted phosphoprotein 1, Bone sialoprotein 1, BSP-1, OPN, and SPP1, is a member of the osteopontin family and a SIBLING glycoprotein. Osteopontin has been classified as T-helper 1 cytokine and thus believed to exacerbate inflammation in several chronic inflammatory diseases, including atherosclerosis. Besides proinflammatory functions, physiologically Osteopontin is a potent inhibitor of mineralization, it prevents ectopic calcium deposits and is a potent inducible inhibitor of vascular calcification. Osteopontin is expressed and secreted by various cells, and has a role in cell adhesion, chemotaxis, prevention of apoptosis, invasion, migration and anchorage-independent growth of tumor cells. Osteopontin recruitment functions of inflammatory cells are thought to be mediated through its adhesive domains, especially the arginine-glycine-aspartate (RGD) sequence that interacts with several integrin heterodimers. Osteopontin has emerged as a potential biomarker and mediator in cardiovascular disease. In the context of atherosclerosis, OPN is generally regarded as a proinflammatory and proatherogenic molecule. However, the role of OPN in vascular calcification (VC), which is closely related to chronic and active inflammation, is that of a negative regulator because it is an inhibitor of calcification and an active inducer of decalcification. Extensive research has demonstrated the pivotal participation of Osteopontin in the regulation of cell signaling which controls neoplastic and malignant transformation. The elevated expression of Osteopontin has been observed in a variety of cancers. It has been linked with tumor metastasis and signifies a poor prognosis for the patient.

Full Name
secreted phosphoprotein 1
  • Scatena M, et al. (2007) Osteopontin: a multifunctional molecule regulating chronic inflammation and vascular disease. Arterioscler Thromb Vasc Biol. 27(11): 2302-9.
  • Johnston NI, et al. (2008) Osteopontin as a target for cancer therapy. Front Biosci. 13: 4361-72.
  • Cho HJ, et al. (2009) Osteopontin: a multifunctional protein at the crossroads of inflammation, atherosclerosis, and vascular calcification. Curr Atheroscler Rep. 11(3): 206-13.
  • Waller AH, et al. (2010) Osteopontin in cardiovascular disease: a potential therapeutic target. Cardiol Rev. 18(3): 125-31.
  • Shevde LA, et al. (2010) Osteopontin: an effector and an effect of tumor metastasis. Curr Mol Med. 10(1): 71-81.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Osteopontin Antibody Rabbit Polyclonal ELISA 10352-RP01
    Anti-Osteopontin Antibody Rabbit Monoclonal ELISA 10352-R014
    Anti-Osteopontin Antibody Rabbit Polyclonal ELISA 10352-RP02
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