Anti-SPARC Antibody

Cat: 10929-RP02
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Anti-SPARC Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-SPARC Antibody
Validated applications
ELISA,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human SPARC
Immunogen
Recombinant Human SPARC protein (Catalog#10929-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human SPARC (rh SPARC; Catalog#10929-H08H; NP_003109.1; Met 1-Ile 303). SPARC specific IgG was purified by human SPARC affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-SPARC Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  
IHC-P 1:500-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-SPARC Antibody (Rabbit Polyclonal antibody) Images
Human Osteonectin / SPARC Immunohistochemistry(IHC) 3558
Immunochemical staining of human SPARC in human skin with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human Osteonectin / SPARC Immunohistochemistry(IHC) 3559
Immunochemical staining of human SPARC in human placenta with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human Osteonectin / SPARC Immunohistochemistry(IHC) 3560
Immunochemical staining of human SPARC in human ovarian cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Anti-SPARC Antibody Alternative Names
Anti-BM-40 Antibody;Anti-ON Antibody;Anti-SPARC Antibody
SPARC Background Information

Secreted protein acidic and rich in cysteine (SPARC), also known as Osteonectin (ON), is a member of the SPARC family. SPARC consists of three domains: a EF-hand domain, a follistatin-like domain and a Kazal-like domain, and each of which has independent activity and unique properties. The activity of SPARC is context- and cell-type-dependent, which is highlighted by the fact that SPARC has shown seemingly contradictory effects on tumor progression in both clinical correlative studies and in animal models. The location of SPARC in the nuclear matrix of certain proliferating cells, but only in the cytosol of postmitotic neurons, indicates potential functions of SPARC as a nuclear protein, which might be involved in the regulation of cell cycle progression and mitosis. It functions not only to modulate cell-cell and cell-matrix interactions, but its de-adhesive and growth inhibitory properties in non-transformed cells have led to studies to assess its role in cancer. Its divergent actions reflect the complexity of this protein, because in certain types of cancers, such as melanomas and gliomas, SPARC is associated with a highly aggressive tumor phenotype, while in others, mainly ovarian, neuroblastomas and colorectal cancers, SPARC may function as a tumor suppressor. Recent studies have also demonstrated a role for SPARC in sensitizing therapy-resistant cancers. Notably, SPARC is linked to human obesity.

Full Name
secreted protein, acidic, cysteine-rich (osteonectin)
References
  • Yan Q, et al. (1999) SPARC, a matricellular glycoprotein with important biological functions. J Histochem Cytochem. 47(12): 1495-506.
  • Brekken RA, et al. (2000) SPARC, a matricellular protein: at the crossroads of cell-matrix. Matrix Biol. 19(7): 569-80.
  • Tai IT, et al. (2008) SPARC in cancer biology: its role in cancer progression and potential for therapy. Drug Resist Updat. 11(6): 231-46.
  • Podhajcer OL, et al. (2008) The role of the matricellular protein SPARC in the dynamic interaction between the tumor and the host. Cancer Metastasis Rev. 27(3): 523-37.
  • Kos K, et al. (2010) SPARC: a key player in the pathologies associated with obesity and diabetes. Nat Rev Endocrinol. 6(4): 225-35.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-SPARC Antibody Rabbit Monoclonal WB,ELISA,IP 50494-R001
    Anti-SPARC Antibody Rabbit Polyclonal ELISA 50494-T16
    Anti-SPARC Antibody Rabbit Polyclonal ELISA 50494-RP01
    Anti-SPARC Antibody Rabbit Polyclonal ELISA 50494-RP02
    Anti-SPARC Antibody Rabbit Monoclonal ELISA 10929-R001
    Anti-SPARC Antibody Rabbit Polyclonal ELISA 10929-RP01
    Anti-SPARC Antibody Mouse Monoclonal ELISA 10929-MM14
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