Mouse OMD / Osteomodulin Protein (His Tag)

OMD / Osteomodulin Protein

OMD / Osteomodulin Protein Product Information

• Synonym: Omd, OSAD, SLRR2C
• Protein Construction: A DNA sequence encoding the mouse OMD ( NP_036180.1 ) ( Met 1 - Ile 423 ) precusor was expressed, with a C-terminal polyhistidine tag
• Source: Mouse
• Expression Host: Human Cells

OMD / Osteomodulin Protein QC Testing

• Purity: > 96 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 21
• Molecular Mass: The secreted recombinant mouse OMD comprises 414 amino acids and has a calculated molecular mass of 48.8 kDa. The apparent molecular mass of the recombinant protein is approximately 60-70 kDa in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: OMD protein

OMD / Osteomodulin Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

OMD / Osteomodulin Protein Description

Mouse Osteomodulin, also known as Osteoadherin, Keratan sulfate proteoglycan osteomodulin, KSPG osteomodulin, OSAD, SLRR2C and OMD, is a secreted protein which belongs to the small leucine-rich proteoglycan (SLRP) family and Class II subfamily. SLRP family proteins are normally found in extracellular matrices, but Osteomodulin / OMD is the only member restricted to mineralized tissues. Osteomodulin / OMD is primarily expressed by osteoblasts and might have a role in regulation of mineralization. Osteomodulin / OMD was primarily present in bone trabeculae and no staining was seen in cartilage. In situ hybridization showed the strongest expression in osteoblasts close to the cartilage/bone interface of the growth plate and lower expression in diaphyseal osteoblasts. Osteomodulin / OMD is a cell binding keratan sulfate proteoglycan which was recently isolated from mineralized bovine bone and subsequently cloned and sequenced. Osteomodulin / OMD may be implicated in biomineralization processes. It has a function in binding of osteoblasts via the alpha (V) beta (3)-integrin. It is likely that Osteomodulin / OMD is an osteoblast maturation marker that is induced by osteoclast activity. Osteomodulin / OMD is also an early marker for terminally differentiated matrix producing osteoblasts.

References

1. Shen,Z. et al., 1999, Matrix Biol  18 (6): 533-42.
2. Clark HF. et al., 2003, Genome Res. 13:2265-70.
3. Humphray S.J. et al., 2004, Nature. 429: 369-74.
4. Onnerfjord P. et al., 2004, J. Biol. Chem. 279:26-33.
5. Rehn, AP.et al., 2006,Biochem Biophys Res Commun.349 (3):1057-64.