OMD / Osteomodulin Antibody (Antigen Affinity Purified)

Mouse OMD / Osteomodulin / Osteoadherin Antibody ( Antigen Affinity Purified )

OMD / Osteomodulin Antibody Product Information

• Immunogen: Recombinant mouse OMD protein ( Catalog#50451-M08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant mouse OMD ( rM OMD ; Catalog#50451-M08H ; NP_036180.1 ; Met 1 - Ile 423 ). OMD specific IgG was purified by mouse OMD affinity chromatography

OMD / Osteomodulin Antibody Usage Guide

• Specificity: Mouse OMD / Osteomodulin
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect mouse OMD in WB. Using a DAB detection system, the detection limit for mouse OMD is approximately 2 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect mouse OMD. The detection limit for mouse OMD is approximately 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

OMD / Osteomodulin Antibody Background

Mouse Osteomodulin, also known as Osteoadherin, Keratan sulfate proteoglycan osteomodulin, KSPG osteomodulin, OSAD, SLRR2C and OMD, is a secreted protein which belongs to the small leucine-rich proteoglycan (SLRP) family and Class II subfamily. SLRP family proteins are normally found in extracellular matrices, but Osteomodulin / OMD is the only member restricted to mineralized tissues. Osteomodulin / OMD is primarily expressed by osteoblasts and might have a role in regulation of mineralization. Osteomodulin / OMD was primarily present in bone trabeculae and no staining was seen in cartilage. In situ hybridization showed the strongest expression in osteoblasts close to the cartilage/bone interface of the growth plate and lower expression in diaphyseal osteoblasts. Osteomodulin / OMD is a cell binding keratan sulfate proteoglycan which was recently isolated from mineralized bovine bone and subsequently cloned and sequenced. Osteomodulin / OMD may be implicated in biomineralization processes. It has a function in binding of osteoblasts via the alpha (V) beta (3)-integrin. It is likely that Osteomodulin / OMD is an osteoblast maturation marker that is induced by osteoclast activity. Osteomodulin / OMD is also an early marker for terminally differentiated matrix producing osteoblasts.

References

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