Best OFP/RFP Protein ( Orange Fluorescence Protein): OFPSparkTM
Best OFP/RFP(Orange/Red Fluorescencent Protein) Protein: OFPSparkTM
DsRed commercial orange Red OFPSparkTM
  • Brightest orange
  • Gene Fusion suitable
  • Superior light stability
  • Intellectual property
Tetramer
Not suitable for fusion protein expression
Monomer Brighter
commercial orange Red vs OFPSparkTM: Key Feature Comparison
  Fluorescence color Extinction coefficient(M-1cm-1) Quantum yield Brightness pKa Structure Maturation rate at 30℃ t1/2bleach(s)
OFPSparkTM Red(orange) 84000 0.69 58.0 6.2 Monomer Fast 4.7
Commercial orange red Red(orange) 49000 0.60 29.4 6.8 Monomer Fast 4.8
Search >10,000 Genes Fused with OFPSparkTM:
Expression-Ready Plasmid, Save Time & Cost
*Simply type in gene molecule name: e.g. PD1
Also available with other tags: OFP, FLAG, His, HA, Myc, Fc
commercial orange Red vs OFPSparkTM: Gene Expression

OFPSparkTM or commercial orange Red expression
in 293 H cell for 48 h

Best RFP (OFPSparkTM) Tag Gene Fusion Expression Validation
Microtubule TUBB Mitochondrial PRDX5 Autophagosomes ATG12
endoplasmic reticulumHSPA5 Nucleus WT1 Endosomes RAB5A
 
OFPSparkTM tag fusion expression in Hela cell for 48 h
Best RFP (OFPSparkTM Protein) : Main Characteristics
Characteristic
Molecular weight, kDa 26
Polypeptide length, aa 231
Fluorescence color Red(orange)
Excitation maximum, nm 549
Emission maximum, nm 566
Quantum yielda 0.69
Extinction coefficient, M-1cm-1 84000
Brightnessb 58.0
Brightness, % of EGFP 197
pKa 6.2
Structure Monomer
Aggregation no
Maturation rate at 30℃ Fast

a. mOrange2 as a reference for quantum yield tested;
b. Brightness is a product of extinction coefficient and
quantum yield, divided by 1 000.

Best RFP (OFPSparkTM) Tag Gene Fusion Expression Vectors

N-OFP Expression Vector


C-OFP Expression Vector

Best RFP (OFPSparkTM Protein) : Maturation Kinetics
24h 48h 72h 96h
 
Photostability
 

T1/2 bleach of OFPSparkTM under 561 nm Sapphire Solid laser is 4.7 s (output power of Sapphire Solid laser is 8 mW).

Other OPF related product

Recombinant OFPSparkTM protein

General Information
Gene Name Synonym:
OFP
Protein Construction
OFPSparkTM is a red(orange) fluorescent protein derived from DsRed(similarity is 83%).OFPSparkTM was expressed with a polyhistide tag at the C-terminus(patent 201510003374.3).
Source: Discosoma sp.
Expression Host: E. coli

SEC-HPLC analysis
A: mOrange2, B: OFPSparkTM
OFPSparkTM detection antibody
Red Fluorescent protein
Fluorescent proteins are members of a structurally homologous class of proteins that share the unique property of being self-sufficient to form a visible wavelength chromophore from a sequence of 3 amino acids within their own polypeptide sequence. It is common research practice for biologists to introduce a gene encoding with an engineered fluorescent protein tag into living cells and subsequently visualize the location and dynamics of the gene product using fluorescence microscopy.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"