Mouse Oncostatin M / OSM Protein (His Tag) (Cytokine)

Oncostatin M Protein Datasheet

Oncostatin M / OSM Protein Product Information

• Synonym: RP23-453B22.1, OncoM
• Protein Construction: A DNA sequence encoding the mature form of mouse OSM (P53347) (Met 1-Arg 206) was expressed, with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

Oncostatin M / OSM Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Asn 25
• Molecular Mass: The secreted recombinant mouse OSM comprises 193 amino acids and has a calculated molecular mass of 22 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 35-40 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: Oncostatin M / OSM protein

Oncostatin M / OSM Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Oncostatin M / OSM Protein Description

Oncostatin M (OSM) is a glycoprotein belonging to the interleukin-6 family of cytokines that has functions mainly in cell growth. This polypeptide of Mr approximately 28,000 is found maily in activated T-lymphocyte cell lines and monocyte, associating with inflammation. Oncostatin M (OSM) is considered as a pleiotropic cytokine that signals through cell surface receptors typeⅠand typeⅡ both of which share the similarity of containing protein gp130 and takes part in many biometabolism processes including liver development, haematopoeisis, inflammation, bone formation and destruction and possibly CNS development. Oncostatin M (OSM) was previoustly identified by its ability to inhibit the growth of cells from melanoma and other solid tumors. It also has been reported that OSM, like LIF, IL-6 and G-CSF, has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells. The human form of OSM is insensitive between pH2 and 11 and resistant to heating for one hour at 56 degree but is not stable at 90 degrees. The human OSM is produced as a precursor containing 252 amino acids, whose first 25 amino acids function as a secretory signal peptide and which on removal yields the soluble 227 amino acid pro-OSM. Removal of the C-teminal most 31 amino acids produces the fully active 196 residue form.

References

1. Modur V. et al., 1997, J Clin Invest. 100 (1): 158-68.
2. Malik N. et al., 1989, Mol Cell Biol. 9 (7): 2847-53.
3. Rose TM. et al., 1991, PNAS. 88 (19): 8641-5.