Anti-OTUB2 Antibody (Rabbit Polyclonal antibody) General Information
Reacts with: Human
Recombinant Human OTUB2 protein (Catalog#13177-H07E)
Produced in rabbits immunized with purified, recombinant Human OTUB2 (rh OTUB2; Catalog#13177-H07E; Q96DC9-1; Met1-His234). OTUB2 specific IgG was purified by Human OTUB2 affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-OTUB2 Antibody (Rabbit Polyclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-OTUB2 Antibody Alternative Names
Anti-C14orf137 Antibody;Anti-OTB2 Antibody;Anti-OTU2 Antibody
OTUB2 Background Information
Otubain 2 (OTUB2) is a member of DUBs that belong to the ovarian tumour (OTU) superfamily of proteins which consists of a five-stranded β-sheet sandwiched in between a small helical amino-terminal region consisting of α1 and α2, and a large helical region comprised of α3-α10. Like other DUBs, otubain 2 (OTUB2) cleaves proteins precisely at the ubiquitin-protein bond so that ubiquitylation process can be reversed and regulated. Otubain 2 (OTUB2)'s active-site cleft is sterically occluded by a novel loop conformation resulting in an oxyanion hole, which consists uniquely of backbone amides. Furthermore, the residues that orient and stabilize the active-site histidine of otubain 2 (OTUB2) are different from other cysteine proteases. This reorganization of the active-site topology provides a possible explanation for the low turnover and substrate specificity of the otubains.
OTU deubiquitinase, ubiquitin aldehyde binding 2
Balakirev MY, et al. (2003) Otubains: a new family of cysteine proteases in the ubiquitin pathway. EMBO Rep 4 (5): 517-22.Nanao MH. (2004) Crystal structure of human otubain 2. EMBO reports. 5: 783-8.