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ORP150 / ORP150 / HYOU1 / HSP12A Antibody, Rabbit MAb

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Human HSP12A Antibody Product Information
Immunogen:Recombinant Human ORP150 / HYOU1 / HSP12A protein (Catalog#11342-H08H)
Clone ID:201
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human ORP150 / HYOU1 / HSP12A (rh ORP150 / HYOU1 / HSP12A; Catalog#11342-H08H; NP_001124463.1; Met 695-Leu 994).
Human HSP12A Antibody Usage Guide
Specificity:Human ORP150 / HYOU1 / HSP12A
No cross-reactivity in ELISA with
Human Fetuin A
Application:WB, ELISA, IHC-P, IP

WB: 5-10 μg/ml

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human HSP12A. The detection limit for Human HSP12A is approximately 0.0195 ng/well.

IHC-P: 1-10 μg/mL

IP: 1-4 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human HSP12A Antibody WB Application Image
Human HSP12A Antibody IHC Application Image
ORP150 / ORP150 / HYOU1 / HSP12A Antibody, Rabbit MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human HSP12A in human cirrhosis with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to hepatocyte.
ORP150 / ORP150 / HYOU1 / HSP12A Antibody, Rabbit MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human HSP12A in human colon carcinoma with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to colonic gland.
Human HSP12A Antibody IP Application Image
Other HSP12A Antibody Products
ORP150/HYOU1/HSP12A (Grp170) Background

Hypoxia up-regulated protein 1, also known as 150 kDa oxygen-regulated protein, 170 kDa glucose-regulated protein, ORP-150, GRP-170 and HYOU1, is a member of the heat shock protein 70 family. Seven members from four different heat shock protein (HSP) families were identified including HYOU1 (ORP150), HSPC1 (HSP86), HSPA5 (Bip), HSPD1 (HSP60), and several isoforms of the two testis-specific HSP70 chaperones HSPA2 and HSPA1L. HYOU1 is highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. It is highly expressed in liver and pancreas. HYOU1 is also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers. HYOU1 has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. It may play a role as a molecular chaperone and participate in protein folding. Suppression of HYOU1 is associated with accelerated apoptosis. It is suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness.

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Catalog: 11342-R201-50
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50 µg
100 µg
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All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"