Anti-ORP150 Antibody (Rabbit Polyclonal antibody) General Information
Reacts with: Human
Recombinant Human HSP12A protein (Catalog#11342-H08H)
Produced in rabbits immunized with purified, recombinant Human HSP12A (rh HSP12A; Catalog#11342-H08H; NP_001124463.1; Met 695-Leu 994). HSP12A specific IgG was purified by human HSP12A affinity chromatography
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-ORP150 Antibody (Rabbit Polyclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-ORP150 Antibody (Rabbit Polyclonal antibody) Images
Immunochemical staining of human HSP12A in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human HSP12A in human breast carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Anti-ORP150 Antibody Alternative Names
Anti-GRP-170 Antibody;Anti-Grp170 Antibody;Anti-HSP12A Antibody;Anti-ORP-150 Antibody;Anti-ORP150 Antibody
ORP150 Background Information
Hypoxia up-regulated protein 1, also known as 150 kDa oxygen-regulated protein, 170 kDa glucose-regulated protein, ORP-150, GRP-170 and HYOU1, is a member of the heat shock protein 70 family. Seven members from four different heat shock protein (HSP) families were identified including HYOU1 (ORP150), HSPC1 (HSP86), HSPA5 (Bip), HSPD1 (HSP60), and several isoforms of the two testis-specific HSP70 chaperones HSPA2 and HSPA1L. HYOU1 is highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. It is highly expressed in liver and pancreas. HYOU1 is also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers. HYOU1 has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. It may play a role as a molecular chaperone and participate in protein folding. Suppression of HYOU1 is associated with accelerated apoptosis. It is suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness.
hypoxia up-regulated 1
Ikeda J., et al.,(1997), Cloning and expression of cDNA encoding the human 150 kDa oxygen-regulated protein, ORP150. Biochem. Biophys. Res. Commun. 230:94-99.Kaneda S., et al., (2000), Production of three distinct mRNAs of 150 kDa oxygen-regulated protein (ORP150) by alternative promoters: preferential induction of one species under stress conditions.J. Biochem. 128:529-538.Takeuchi S., (2006), Molecular cloning, sequence, function and structural basis of human heart 150 kDa oxygen-regulated protein, an ER chaperone.Protein J. 25:517-528.