Rat OPCML Gene cDNA Clone / ORF Clone

OPCML cDNA Clone Datasheet

OPCML cDNA Clone Product Information

• Gene Bank Ref.ID: NM_053848.1
• cDNA Size: 1038
• Gene Synonym: Opcml
• Source: Rat

OPCML cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus opioid binding protein/cell adhesion molecule-like DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 57 G/C not causing the amino acid variation.

OPCML cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

OPCML cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

OPCML cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGGTCT GTGGGTACCT GTTCCTGCCC TGGAAGTGCC TCGTGGTCGT GTCTCTCAGG
61       CTGCTGTTCC TTGTACCCAC AGGAGTGCCG GTGCGCAGCG GAGATGCCAC CTTTCCCAAA
121      GCTATGGACA ACGTGACGGT CCGGCAGGGA GAGAGCGCCA CCCTCAGGTG TACCATAGAT
181      GACCGGGTCA CCAGAGTAGC CTGGCTAAAC CGCAGCACAA TCCTCTACGC TGGGAATGAC
241      AAGTGGTCCA TAGACCCTCG AGTGATCATC TTGGTCAACA CGCCTACCCA GTACAGTATC
301      ATGATCCAGA ATGTGGATGT TTATGACGAA GGTCCGTACA CCTGCTCTGT GCAGACAGAC
361      AATCACCCCA AAACCTCCCG GGTCCACCTC ATAGTGCAAG TTCCTCCCCA GATAATGAAC
421      ATCTCTTCAG ACATTACTGT GAATGAGATA AGCAGTGTGA CCTTGTTATG TCTCGCAATT
481      GGCAGACCAG AACCAACAGT GACATGGCGA CACCTGTCAG TCAAGGAAGG CCAGGGCTTT
541      GTGAGTGAAG ATGAATACCT GGAAATCTCA GACATCAAAC GCGACCAATC TGGAGAGTAT
601      GAGTGCAGCG CCTTGAATGA TGTCGCTGCA CCTGATGTTC GGAAAGTAAA AATCACTGTA
661      AACTATCCTC CCTATATCTC AAAAGCGAAG AACACTGGCG TTTCAGTAGG CCAGAAGGGC
721      ATCCTGAGCT GTGAAGCCTC TGCTGTCCCC ATGGCTGAAT TCCAGTGGTT CAAGGAAGAT
781      ACCAGGTTAG CCACTGGCCT GGATGGCGTG AGAATTGAGA ACAAAGGCCG CATATCCACT
841      TTGACTTTCT TCAATGTCTC AGAGAAGGAT TATGGGAACT ATACCTGTGT GGCCACAAAC
901      AAGCTTGGGA ACACCAATGC CAGCATCACC CTGTATGGGC CTGGAGCAGT CATTGATGGT
961      GTAAACTCGG CCTCTAGAGC ACTGGCTTGT CTCTGGCTCT CAGGGACCTT CTTTGCCCAC
1021     TTCTTCATCA AGTTTTGA

 

Quality Control:cDNA sequencing results is available upon request.