Cynomolgus monkey OLR1 Gene cDNA Clone / ORF Clone

OLR1 cDNA Clone Datasheet

OLR1 cDNA Clone Product Information

• cDNA Size: 822
• Gene Synonym: OLR1
• Source: Cynomolgus monkey

OLR1 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) oxidized low density lipoprotein (lectin-like) receptor 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 149 G/T resulting in the amino acid Gly substitution by Val and 178 T/C，372 C/T not causing the amino acid variation.
  Please check the sequence information before order.

OLR1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

OLR1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

OLR1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACTTTTG ATGACCTAAA GATCCAGACT ATGAAGGACC AGCCTGATGA GAAGTCAAAT
61       GGAAAAAAAG CTAAAGGCCT TCAGTTTCTT TATTCTCCAT GGTGGTGCCT GGTTGCTGTG
121      ACTCTGGCAG TCCTCTGCCT GGGATTAGTA GTGACCATTA TGGTGCTGGG CATGCAACTG
181      TCCCAGGTGT CTAACCTCCT AAAGCAACAG CAAACAAACC TAACTCACCA GAAAAATAAA
241      CTGGAGGGAC AGATCTCAGC CCGGCAACAA GCAGAAGAAG CTTCACAGGA ATCACAAAAC
301      GAACTCAAGG AAATGATAGA AACCCTTGCT TGGAAGCTGA ATGAGAAATC CAAAGAGCAA
361      ATGGAACTTC ATCACCAGAA TCTGAATCTC CAAGAAACAC TGAAGAGAGT AGCAAATTGT
421      TCAGCTCCTT GTCCGCAAGA CTGGATCTGG CATGAAGAAA ACTGTTACCT ATTTTCCACG
481      GGCTCATTTA ACTGGGAAAA GAGCCAAGAG AAGTGCTTGT CTTTGGATGC CAAGTTGCTG
541      AAAATTAATA GTACAGCTGA TCTGGACTTC ATCCAGCAAG CAATTTCCTA TTCCAGTTTC
601      CCATTCTGGA TGGGGCTGTC TCGGAGAAAC CCCAGCTATC CATGGCTCTG GGAGGACGGT
661      TCTCCTTTGA TGCCCCACTT ATTTAGAATC CGAGGTGCTG TTTCCCAGAC ATACCCTTCA
721      GGTACCTGTG CATATATACA GCGAGGAGCT GTTTATGCGG AAAACTGCAT TTTAGCTGCC
781      TTCAGTATAT GTCAGAAGAA GGCAAACCTA AGAGCACAGT GA

 

Quality Control:cDNA sequencing results is available upon request.