OLR1 / LOX1 Protein (His Tag)

Oxidized low density lipoprotein (lectin-like) receptor 1 Protein Datasheet

OLR1 / LOX1 Protein Product Information

• Synonym: OLR1 ,CLEC8A, LOX1, LOXIN, SCARE1, SLOX1
• Protein Construction: A DNA sequence encoding the human OLR1 (P78380) extracellular domain (Ser 61-Gln 273) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: Human Cells

OLR1 / LOX1 Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His
• Molecular Mass: The recombinant human OLR1 consists of 229 amino acids and has a calculated molecular mass of 26.5 kDa. It exists as a disulfide-linked homodimer. As a result of glycosylation, the apparent molecular mass of rhOLR1 is approximately 30-35 kDa in SDS-PAGE under reducing conditions, and 60-70 KDa in non-reduced SDS-PAGE.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: OLR1 / LOX1 protein

OLR1 / LOX1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

OLR1 / LOX1 Protein Description

Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1) also known as lectin-type oxidized LDL receptor 1 (LOX-1) is a receptor protein which belongs to the C-type lectin superfamily. Lectin-like oxidized LDL receptor-1 (LOX-1) was isolated from an aortic endothelial cell, and recently it has been discovered in macrophages and vascular smooth muscle cells in artery vessels. The expression of LOX-1 is inducted by inflammatory stimuli, so LOX-1 is thought to be involved in the development of atherosclerotic lesions. It is an important cell surface receptor for the progression of atherosclerosis. LOX-1 is a multi-ligand receptor originally identified as the endothelial oxidized LDL receptor. LOX-1 expression is also induced in smooth muscle cells in response to proinflammatory and oxidative stimuli. LOX-1 may play an important role in the progression of vulnerable carotid plaque and might regulate vulnerable plaque formation in cooperation with MMPs and TIMP-2.

References

1. Hinagata J. et al., 2006, Cardiovasc Res. 69 (1): 263-71.
2. Melan MA. et al., 1994, Plant Physiol. 105 (1): 385-393.
3. Saito A. et al., 2010, Acta Neurochir (Wien). 152 (4): 589-95.