Human OLFM4 Gene cDNA Clone / ORF Clone

OLFM4 cDNA Clone Datasheet

OLFM4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_006418.3
• cDNA Size: 1532
• Gene Synonym: GC1; OLM4; OlfD; GW112; hGC-1; hOLfD; UNQ362; KIAA4294; bA209J19.1; OLFM4
• Source: Human

OLFM4 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens olfactomedin 4 DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutation: 1A/T not causing the amino acid variation and the bottom 3T deletion mutation.

OLFM4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

OLFM4 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

OLFM4 cDNA Clone Sequence and Quality Control

Sequence:

1        TTGAGGCCCG GCCTCTCATT TCTCCTAGCC CTTCTGTTCT TCCTTGGCCA AGCTGCAGGG
61       GATTTGGGGG ATGTGGGACC TCCAATTCCC AGCCCCGGCT TCAGCTCTTT CCCAGGTGTT
121      GACTCCAGCT CCAGCTTCAG CTCCAGCTCC AGGTCGGGCT CCAGCTCCAG CCGCAGCTTA
181      GGCAGCGGAG GTTCTGTGTC CCAGTTGTTT TCCAATTTCA CCGGCTCCGT GGATGACCGT
241      GGGACCTGCC AGTGCTCTGT TTCCCTGCCA GACACCACCT TTCCCGTGGA CAGAGTGGAA
301      CGCTTGGAAT TCACAGCTCA TGTTCTTTCT CAGAAGTTTG AGAAAGAACT TTCCAAAGTG
361      AGGGAATATG TCCAATTAAT TAGTGTGTAT GAAAAGAAAC TGTTAAACCT AACTGTCCGA
421      ATTGACATCA TGGAGAAGGA TACCATTTCT TACACTGAAC TGGACTTCGA GCTGATCAAG
481      GTAGAAGTGA AGGAGATGGA AAAACTGGTC ATACAGCTGA AGGAGAGTTT TGGTGGAAGC
541      TCAGAAATTG TTGACCAGCT GGAGGTGGAG ATAAGAAATA TGACTCTCTT GGTAGAGAAG
601      CTTGAGACAC TAGACAAAAA CAATGTCCTT GCCATTCGCC GAGAAATCGT GGCTCTGAAG
661      ACCAAGCTGA AAGAGTGTGA GGCCTCTAAA GATCAAAACA CCCCTGTCGT CCACCCTCCT
721      CCCACTCCAG GGAGCTGTGG TCATGGTGGT GTGGTGAACA TCAGCAAACC GTCTGTGGTT
781      CAGCTCAACT GGAGAGGGTT TTCTTATCTA TATGGTGCTT GGGGTAGGGA TTACTCTCCC
841      CAGCATCCAA ACAAAGGACT GTATTGGGTG GCGCCATTGA ATACAGATGG GAGACTGTTG
901      GAGTATTATA GACTGTACAA CACACTGGAT GATTTGCTAT TGTATATAAA TGCTCGAGAG
961      TTGCGGATCA CCTATGGCCA AGGTAGTGGT ACAGCAGTTT ACAACAACAA CATGTACGTC
1021     AACATGTACA ACACCGGGAA TATTGCCAGA GTTAACCTGA CCACCAACAC GATTGCTGTG
1081     ACTCAAACTC TCCCTAATGC TGCCTATAAT AACCGCTTTT CATATGCTAA TGTTGCTTGG
1141     CAAGATATTG ACTTTGCTGT GGATGAGAAT GGATTGTGGG TTATTTATTC AACTGAAGCC
1201     AGCACTGGTA ACATGGTGAT TAGTAAACTC AATGACACCA CACTTCAGGT GCTAAACACT
1261     TGGTATACCA AGCAGTATAA ACCATCTGCT TCTAACGCCT TCATGGTATG TGGGGTTCTG
1321     TATGCCACCC GTACTATGAA CACCAGAACA GAAGAGATTT TTTACTATTA TGACACAAAC
1381     ACAGGGAAAG AGGGCAAACT AGACATTGTA ATGCATAAGA TGCAGGAAAA AGTGCAGAGC
1441     ATTAACTATA ACCCTTTTGA CCAGAAACTT TATGTCTATA ACGATGGTTA CCTTCTGAAT
1501     TATGATCTTT CTGTCTTGCA GAAGCCCCAG AA 

Quality Control:cDNA sequencing results is available upon request.