- EGFR Signaling Pathway
- TGF-beta Signaling
- Canonical Wnt Signaling
- non-Canonical Wnt Signaling
- Notch Signaling
- p53 Pathway
- NF-kB Pathway
- Cytokine Signaling
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OLFM4 / GW112 Antibody Datasheet
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OLFM4 / GW112 Antibody Product Information
Recombinant Human OLFM4 / GW112 protein (Catalog#11639-H08H)
|Antibody Type :||Mouse Monoclonal Antibody ( Mouse mAb Service Platform )|
Clone ID :
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.|
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human OLFM4 / GW112 (rh OLFM4 / GW112; Catalog#11639-H08H; NP_006409.3; Met 1-Gln 510).
OLFM4 / GW112 Antibody Usage Guide
Human OLFM4 / GW112
|Flow Cytometry :||
Flow cytometric analysis of Human OLFM4 expression in DU145 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-OLFM4 (11639-MM12, 1 μg/test), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.Flow cytometry was performed on a BD FACSCalibur flow cytometry system
Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
|Direct ELISA :||This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human OLFM4.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
OLFM4 / GW112 Antibody Related Products & Topics
OLFM4 / GW112 Antibody Related Areas:
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|OLFM4/GW112||Human||OLFM4/GW112 Protein, Recombinant||11639-H08H|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|Human OLFM4/GW112||WB, ELISA||OLFM4 / GW112 Antibody||11639-MM02|
|Human OLFM4/GW112||ELISA, FCM||OLFM4 / GW112 Antibody||11639-MM12|
OLFM4 / GW112 Antibody Background
- Zhang J., et al., 2002, Gene 283: 83-93.
- Zhang X., et al., 2004, Cancer Res. 64: 2474-2481.
- Kobayashi D., 2007, et al., Cancer Sci. 98: 334-340.
- Kobayashi, D.et al., 2007, Cancer Sci. 98 (3): 334-40.
- van der Flier, L.G. et al., 2009, Gastroenterology. 137 (1): 15-7.