Human OAF Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13761-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

OAF cDNA Clone Datasheet

OAF cDNA Clone Product Information

Gene Bank Ref.ID:	BC047726
cDNA Size:	822
Gene Synonym:	MGC52117; NS5ATP13TP2; OAF
Source:	Human

OAF cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens OAF homolog (Drosophila) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

OAF cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

OAF cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

OAF cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGCCTTC CCGGGGTACC CCTGGCGCGC CCTGCGCTGC TGCTGCTGCT GCCGCTGCTC
61       GCGCCGCTGC TGGGAACGGG TGCGCCGGCC GAGCTGCGGG TCCGCGTGCG GCTGCCGGAC
121      GGCCAGGTGA CCGAGGAGAG CCTGCAGGCG GACAGCGACG CGGACAGCAT CAGCCTCGAG
181      CTGCGCAAGC CCGACGGCAC CCTCGTCTCC TTCACCGCCG ACTTCAAGAA GGATGTGAAG
241      GTCTTCCGGG CCCTGATCCT GGGGGAGCTG GAGAAGGGGC AGAGTCAGTT CCAGGCCCTC
301      TGCTTTGTCA CCCAGCTGCA GCACAATGAG ATCATCCCCA GTGAGGCCAT GGCCAAGCTC
361      CGGCAGAAAA ATCCCCGGGC AGTGCGGCAG GCGGAGGAGG TTCGGGGTCT GGAGCATCTG
421      CACATGGATG TCGCTGTCAA CTTCAGCCAG GGGGCCCTGC TGAGCCCCCA TCTCCACAAC
481      GTGTGTGCCG AGGCCGTGGA TGCCATCTAC ACCCGCCAGG AGGATGTCCG GTTCTGGCTG
541      GAGCAAGGTG TGGACAGTTC TGTGTTCGAG GCTCTGCCCA AGGCCTCAGA GCAGGCGGAG
601      CTGCCTCGCT GCAGGCAGGT GGGGGACCAC GGGAAGCCCT GCGTCTGCCG CTATGGCCTG
661      AGCCTGGCCT GGTACCCCTG CATGCTCAAG TACTGCCACA GCCGCGACCG GCCCACGCCC
721      TACAAGTGTG GCATCCGCAG CTGCCAGAAG AGCTACAGCT TCGACTTCTA CGTGCCCCAG
781      AGGCAGCTGT GTCTCTGGGA TGAGGATCCC TACCCAGGCT AG

Quality Control:cDNA sequencing results is available upon request.