Human Noggin Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10267-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_005450.4

cDNA Size:    699

Synonym:   NOG, SYM1, SYNS1

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens noggin DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence.

Shipping

10 μg Plasmid DNA on Whatman FTA elute card Whatman Cat. No.: WB120410.
The product will be shipped at room temperature and will use 3-5 days on road typically.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGGAGCGCT GCCCCAGCCT AGGGGTCACC CTCTACGCCC TGGTGGTGGT CCTGGGGCTG
61       CGGGCGACAC CGGCCGGCGG CCAGCACTAT CTCCACATCC GCCCGGCACC CAGCGACAAC
121      CTGCCCCTGG TGGACCTCAT CGAACACCCA GACCCTATCT TTGACCCCAA GGAAAAGGAT
181      CTGAACGAGA CGCTGCTGCG CTCGCTGCTC GGGGGCCACT ACGACCCAGG CTTCATGGCC
241      ACCTCGCCCC CCGAGGACCG GCCCGGCGGG GGCGGGGGTG CAGCTGGGGG CGCGGAGGAC
301      CTGGCGGAGC TGGACCAGCT GCTGCGGCAG CGGCCGTCGG GGGCCATGCC GAGCGAGATC
361      AAAGGGCTAG AGTTCTCCGA GGGCTTGGCC CAGGGCAAGA AGCAGCGCCT AAGCAAGAAG
421      CTGCGGAGGA AGTTACAGAT GTGGCTGTGG TCGCAGACAT TCTGCCCCGT GCTGTACGCG
481      TGGAACGACC TGGGCAGCCG CTTTTGGCCG CGCTACGTGA AGGTGGGCAG CTGCTTCAGT
541      AAGCGCTCGT GCTCCGTGCC CGAGGGCATG GTGTGCAAGC CGTCCAAGTC CGTGCACCTC
601      ACGGTGCTGC GGTGGCGCTG TCAGCGGCGC GGGGGCCAGC GCTGCGGCTG GATTCCCATC
661      CAGTACCCCA TCATTTCCGA GTGCAAGTGC TCGTGCTAG

Quality Control: cDNA sequencing results is available upon request.

Noggin related areas, pathways, and other information

Cytokine

TGF-beta