Mouse NCSTN Gene cDNA Clone / ORF Clone

NCSTN cDNA Clone Datasheet

NCSTN cDNA Clone Product Information

• Gene Bank Ref.ID: NM_021607.3
• cDNA Size: 2127
• Gene Synonym: Nct; Aph2; AA727311; D1Dau13e; Kiaa0253; mKIAA0253; 9430068N19Rik; Ncstn
• Source: Mouse

NCSTN cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus nicastrin DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutations:62 C/T resulting in the amino acid Ser substitution by Phe, 2033 C/T resulting in the amino acid Thr substitution by Ile, 2038 G/A resulting in the amino acid Val substitution by Ile, 219 T/C, 753 C/T, 1074 T/C and 1609 T/C not causing the amino acid variation.

NCSTN cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NCSTN cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NCSTN cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTACGA CTAGGGGCGG CTCTGGGCCT GACCCAGGAA GTCGGGGTCT TCTTCTTCTG
61       TTTTTTTCCG TGGTACTGGC AGGATTGTGT GGGGGAAACT CAGTGGAGAG GAAAATCTAC
121      ATTCCCTTAA ATAAAACAGC TCCTTGTGTC CGCCTGCTCA ACGCCACTCA TCAGATTGGC
181      TGCCAGTCTT CAATTAGTGG GGATACAGGG GTTATCCACG TAGTGGAGAA AGAAGAAGAC
241      CTGAAGTGGG TGTTGACCGA TGGCCCCAAC CCCCCTTACA TGGTTCTGCT GGAGGGCAAG
301      CTCTTCACCA GAGATGTAAT GGAGAAGCTG AAGGGAACAA CCAGTAGAAT CGCTGGTCTT
361      GCCGTGACTC TAGCCAAGCC CAACTCAACT TCAAGCTTCT CTCCTAGTGT GCAGTGCCCA
421      AATGATGGGT TTGGTATTTA CTCCAACTCC TACGGGCCAG AGTTTGCTCA CTGCAAGAAA
481      ACACTGTGGA ATGAACTGGG CAACGGCTTG GCTTATGAAG ACTTTAGTTT CCCCATCTTT
541      CTTCTTGAAG ATGAGAACGA AACCAAGGTC ATCAAGCAGT GCTATCAAGA TCACAACCTG
601      GGTCAGAATG GCTCTGCACC AAGCTTCCCA TTGTGTGCTA TGCAGCTCTT CTCACACATG
661      CACGCCGTCA TCAGCACTGC CACCTGCATG CGGCGCAGCT TCATCCAGAG CACCTTCAGC
721      ATCAACCCAG AAATCGTCTG TGACCCCTTA TCTGACTACA ACGTATGGAG CATGCTTAAG
781      CCTATAAATA CATCTGTGGG ACTAGAACCT GACGTCAGGG TTGTGGTTGC GGCCACACGG
841      CTGGATAGCC GGTCCTTTTT CTGGAATGTG GCCCCAGGGG CTGAAAGTGC TGTAGCCTCC
901      TTTGTCACTC AGCTGGCTGC AGCTGAAGCT TTGCACAAGG CACCTGATGT GACCACTCTA
961      TCCCGAAATG TGATGTTTGT CTTCTTCCAG GGGGAAACTT TTGACTACAT TGGCAGCTCA
1021     CGGATGGTCT ATGATATGGA GAACGGCAAG TTTCCCGTGC GGCTCGAGAA CATCGACTCC
1081     TTCGTGGAGC TGGGACAGGT GGCCCTAAGA ACTTCACTAG ATCTCTGGAT GCACACAGAT
1141     CCCATGTCTC AGAAAAATGA GTCTGTGAAA AACCAGGTGG AGGATCTTCT GGCCACTCTG
1201     GAGAAGAGCG GTGCTGGTGT CCCTGAAGTT GTCCTGAGGA GACTGGCCCA GTCCCAGGCC
1261     CTTCCACCTT CGTCTCTACA ACGATTTCTT CGGGCTCGAA ACATCTCTGG CGTGGTCCTG
1321     GCTGACCACT CTGGCTCCTT CCACAATCGG TATTACCAGA GCATTTATGA CACGGCTGAG
1381     AACATTAATG TGACCTATCC TGAGTGGCAG AGCCCAGAAG AGGACCTCAA CTTTGTGACA
1441     GACACTGCCA AGGCACTGGC GAATGTGGCC ACAGTGCTGG CGCGTGCACT GTATGAGCTT
1501     GCAGGAGGAA CCAACTTCAG CAGCTCCATC CAGGCTGATC CCCAGACAGT TACACGTCTG
1561     CTCTATGGGT TCCTGGTTAG AGCTAACAAC TCATGGTTTC AGTCGATCCT GAAACATGAC
1621     CTAAGGTCCT ATTTGGATGA CAGGCCTCTT CAACACTACA TCGCCGTCTC CAGCCCTACC
1681     AACACGACTT ACGTTGTGCA GTACGCCTTG GCAAACCTGA CTGGCAAGGC GACCAACCTC
1741     ACCCGAGAGC AGTGCCAGGA TCCAAGTAAA GTCCCAAATG AGAGCAAGGA TTTATATGAA
1801     TACTCGTGGG TACAAGGCCC TTGGAATTCC AACAGGACAG AGAGGCTCCC ACAGTGTGTG
1861     CGCTCCACAG TGCGACTGGC CAGGGCCTTG TCCCCTGCCT TTGAACTGAG TCAGTGGAGC
1921     TCCACAGAAT ACTCTACGTG GGCGGAGAGC CGCTGGAAAG ACATCCAAGC TCGGATATTC
1981     CTAATTGCCA GCAAAGAGCT TGAGTTCATC ACGCTGATCG TGGGCTTCAG CATCCTTATC
2041     TTCTCTCTCA TCGTCACCTA CTGCATCAAT GCCAAAGCCG ACGTCCTTTT CGTTGCTCCC
2101     CGAGAGCCAG GAGCTGTGTC TTACTGA

 

Quality Control:cDNA sequencing results is available upon request.