Nicastrin / NCSTN Antibody

Nicastrin / NCSTN Antibody Datasheet

Nicastrin / NCSTN Antibody Product Information

• Immunogen: Recombinant Human Nicastrin / NCSTN Protein (Catalog#11183-H08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 007
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human Nicastrin / NCSTN (rh Nicastrin / NCSTN; Catalog#11183-H08H; NP_056146.1; Met 1- Glu 669).

Nicastrin / NCSTN Antibody Usage Guide

• Specificity: Human Nicastrin / NCSTN
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human NCSTN in WB. Using a DAB detection system, the detection limit for Human NCSTN is approximately 10 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human NCSTN. The detection limit for Human NCSTN is approximately 0.0049 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Nicastrin / NCSTN Antibody Background

Nicastrin, also known as NCSTN, is a single-pass type I  membrane protein which belongs to the nicastrin family. Nicastrin was named after the Italian village Nicastro, reflecting the fact that Alzheimer's disease was described in 1963 after studying descendants of an extended family originating in the village of Nicastro that had Familial Alzheimer's Disease (FAD). Nicastrin is a protein that is part of the gamma secretase protein complex, which is one of the proteases involved in processing amyloid precursor protein (APP) to the short Alzheimer's disease-associated peptide amyloid beta. Nicastrin may represent a stabilizing cofactor required for the assembly of the gamma-secretase complex. The other proteins in the complex are presenilin, which is the catalytically active component of the complex, APH-1, and PEN-2. Nicastrin itself is not catalytically active, but instead promotes the maturation and proper trafficking of the other proteins in the complex, all of which undergo significant post-translational modification before becoming active in the cell. Nicastrin has also been identified as a regulator of neprilysin, an enzyme involved in the degradation of amyloid beta fragment. 

References

1. Feldman, RG. et al., 1963, Neurology 13: 811-24. 
2. Satoh, J. et al., 2001, Neuropathology. 21: 115-22.
3. Zhang, YW. et al., 2005, J. Biol. Chem. 280 (17): 17020-6. 
4. Kaether, C. et al., 2006, Neurodegener Dis 3 (4-5): 275-83.
5. Chi, A. et al., 2006, J. Proteome Res. 5: 3135-44.