Anti-Neutrophil Elastase Antibody

Cat: 50204-RP02
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Anti-Neutrophil Elastase Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Neutrophil Elastase Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Mouse
Specificity
Mouse Neutrophil Elastase
Immunogen
Recombinant Mouse ELA2 protein (Catalog#50204-M08H)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse ELA2 (rM ELA2; Catalog#50204-M08H; NP_056594.2; Met 1-Arg 260). ELA2 specific IgG was purified by mouse ELA2 affinity chromatography .
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Neutrophil Elastase Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Neutrophil Elastase Antibody Alternative Names
Anti-Ela2 Antibody;Anti-F430011M15Rik Antibody;Anti-NE Antibody
Neutrophil Elastase Background Information

Neutrophil elastase, also known as Elastase-2, Bone marrow serine protease, Medullasin, ELANE, and ELA2, is a serine proteinase in the same family as chymotrypsin and has broad substrate specificity. Secreted by neutrophils during inflammation, it destroys bacteria and host tissue. As with other serine proteinases, ELANE / ELA2 contains a charge relay system composed of the catalytic triad of histidine, aspartate, and serine residues that are dispersed throughout the primary sequence of the polypeptide but that are brought together in the three dimension conformation of the folded protein. ELANE / ELA2 is an important protease enzyme that when expressed aberrantly can cause emphysema or emphysematous changes. This involves breakdown of the lung structure and increased airspaces. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. ELANE / ELA2 hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. ELANE / ELA2 may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. ELANE / ELA2 degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Defects in ELANE are a cause of cyclic haematopoiesis (CH), also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Defects in ELANE are also the cause of autosomal dominant severe congenital neutropenia type 1 (SCN1) which is a heterogeneous disorder of hematopoiesis.

Full Name
elastase, neutrophil expressed
References
  • Nakamura H., et al.,(1987), Nucleotide sequence of human bone marrow serine protease (medullasin) gene. Nucleic Acids Res. 15:9601-9601.
  • Takahashi H., et al., (1988), Structure of the human neutrophil elastase gene.J. Biol. Chem. 263:14739-14747.
  • Farley D., et al.,(1989), The human neutrophil elastase gene. Analysis of the nucleotide sequence reveals three distinct classes of repetitive DNA.Biol. Chem. Hoppe-Seyler 370:737-744.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Neutrophil Elastase Antibody Rabbit Polyclonal WB 50204-T32
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