+86-400-890-9989
Product Catalog
Research Topics
Rat Nephrin Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| RG80247-G |
|
YES |  |
|
|
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
 |
|||
Nephrin cDNA Clone Datasheet
Nephrin cDNA Clone Product Information
| Gene Bank Ref.ID: | NM_022628.1 |
|
cDNA Size: |
3759 |
| Gene Synonym: |
Nphs1; nephrin |
| Source: | Rat |
Nephrin cDNA Clone Description
|
cDNA Description: |
ORF Clone of Rattus norvegicus nephrosis 1, congenital, Finnish type DNA. |
| Vector: | Cloning Vector: pGEM-T Vector
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Identical with the Gene Bank Ref. ID sequence except for the point mutations: 56 C/T, 137 A/G, 1669 T/C and 1712 T/G resulting in the amino acid thr substitution by met, gln substitution by arg, phe substitution by leu and 531A/G，942C/T not causing the amino acid variation. |
Nephrin cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
Nephrin cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
Nephrin cDNA Clone Sequence and Quality Control
Sequence:
1 ATGGGCGCTA AGAGAGTCAC TGTCAGAGGT GCCCGGACAA GCCCAATACA CAGAATGTCC 61 AGTTTGACTC CCCTGCTGCT CATGGGAATG CTGACCTCAG GCCTGGCCGA GTCGCCAGTC 121 CCCACCTCAG CACCTCGAGG CTTCTGGGCT CTGTCTGAAA ACCTGACTGC GGTGGAAGGG 181 ACAACAGTTA AGCTATGGTG CGGTGTCAGG GCCCCTGGCA GTGTGGTGCA GTGGGCTAAG 241 GATGGGCTGC TTCTGGGTCC AAACCCGAAG ATGCCAGGCT TCCCGAGGTA CAGCCTGGAA 301 GGAGATCGTG CTAAAGGCGA GTTCCACCTG CTTATTGAAG CCTGTGACCT CAGTGATGAC 361 GCAGAGTATG AATGCCAAGT CGGCCGCTCA GAGTTGGGTC CCGAGCTTGT GTCTCCTAAA 421 GTAATCCTCT CCATTCTAGT TTCCCCCAAG GTGCTTCTGT TGACCCCCGA GGCAGGAAGC 481 ACAGTGACCT GGGTAGCTGG GCAGGAGTAT GTGGTCACCT GTGTGTCTGG GGATGCAAAA 541 CCAGCACCTG ACATCACCTT CATCCAGAGT GGACGAACTA TATTGGACGT CTCCTCCAAT 601 GTGAATGAGG GATCAGAGGA GAAACTCTGC ATCACAGAGG CCGAAGCCAG GGTGATACCC 661 CAGAGCTCGG ATAACGGGCA GTTACTGGTC TGTGAGGGTT CCAACCCAGC TTTGGACACT 721 CCCATAAAGG CTTCATTCAC CATGAATATT CTGTTTCCCC CAGGACCTCC TGTCATTGAT 781 TGGCCAGGCC TGAATGAGGG GCATGTGAGG GCAGGGGAGA ACCTGGAGCT GCCCTGCACA 841 GCCAGAGGTG GCAATCCACC TGCTACCCTG CAGTGGCTGA AGAACGGTAA ACCAGTGTCC 901 ACAGCCTGGG GCACCGAGCA TGCCCAGGCA GTGGCCCACA GTGTGCTGGT GATGACTGTA 961 CGACCTGAAG ACCATGGAGC TCGGCTCAGC TGTCAGTCCT ACAACAGCGT GTCTGCAGGG 1021 ACCCAGGAGA GAAGCATCAC ACTACAGGTC ACCTTTCCCC CAAGCGCCAT TACCATCCTG 1081 GGATCTGTAT CACAATCGGA GAACAAGAAC GTGACCCTTT GCTGCCTGAC CAAGTCCAGT 1141 CGCCCACGGG TCCTGCTGCG ATGGTGGTTG GGTGGACGGC AGCTGCTGCC CACAGATGAG 1201 ACAGTCATGG ATGGCCTGCA TGGTGGCCAC ATCTCCATGT CCAATCTCAC ATTCTTGGTG 1261 CGGAGAGAAG ACAATGGCCT GCCCCTCACC TGTGAAGCCT TCAGTGACGC CTTCAGCAAG 1321 GAGACCTTCA AGAAGTCACT CACCTTGAAT GTGAAATACC CTGCCCAGAA GCTGTGGATT 1381 GAGGGGCCCC CAGAGGGACA GTACATCCGG ACTGGGACTC GGGTGAGGCT GGTATGCTTG 1441 GCCATCGGAG GCAACCCAGA CCCCTCCCTC ATCTGGTTTA AGGATTCACG TCCGGTGAGC 1501 GAGCCCCGGC AGCCCCAGGA GCCCCGGCGT GTGCAGCTGG GCAGTGTGGA GAAGTCCGGG 1561 AGCACTTTCT CCCGCGAGCT GGTGTTGATC ATAGGTCCGC CGGACAACCG AGCCAAGTTC 1621 TCCTGCAAGG CGGGTCAGCT CAGTGCGTCT ACGCAGCTGG TGGTGCAGCT CCCCCCAACC 1681 AACCTGACCA TCCTGGCCAA CTCGTCCGCG CGGCGCCCAG GCGACGCCTT GAACTTGACC 1741 TGCGTCAGCA TCAGCAGCAA CCCCCCAGTC AACTTGTCTT GGGACAAGGA AGGAGAGAGG 1801 CTGGAAGATG TGGCTGCAAA ACCCCAGAGT GCACCGTTCA AAGGCTCCGC TGCATCCAGG 1861 AGTGTTTTTC TCAGAGTGTC ATCCCGAGAC CACGGTCAAC GGGTCACCTG CCGGGCCCAC 1921 AGCGAGGCAC TCCGTGAAAC CGTGAGCTCC TTCTACCGCT TCAATGTGCT GTATCCTCCA 1981 GAATTCCTGG GGGAGCAAGT CCGGGCAGTG ACCGTGGTGG AGCAGGGCCA GGTGCTGCTG 2041 CCGGTGTCGG TGTCCGCTAA CCCCGCCCCC GAGGCCTTCA ACTGGACCTT CCGAGGCTAC 2101 CGCCTCAGCC CAGCTGGGGG TCCCCGGCAC CGTATCCTGT CTGGAGGGGC TCTGCAGCTG 2161 TGGAATGTGA CCCGAGCTGA CGATGGCTTT TATCAGCTGC ACTGCCAGAA CTCAGAGGGC 2221 ACCGCTGAGG CGCTGTTGAA GCTGGACGTG CATTATGCTC CCACCATCCG TGCCCTCCGG 2281 GACCCTACTG AGGTGAATGT TGGGGGTTCT GTGGACATAG TCTGCACCGT TGACGCCAAT 2341 CCCATCCTCC CAGAGATGTT CAGCTGGGAG AGACTGGGAG AAGAAGAGGA GGATCTGAAC 2401 CTGGACGACA TGGAGAAAGT TTCCAAGGGA TCCACGGGGC GTCTGCGGAT TCGCCAAGCC 2461 AAGCTATCCC AGGCTGGTGC CTACCAGTGC ATCGTGGACA ATGGGGTGGC TCCTGCAGCC 2521 AGAGGACTGG TTCGTCTTGT CGTCCGATTT GCTCCCCAGG TGGATCAGCC TACTCCCCTA 2581 ACAAAAGTGG CTGCCGCTGG GGACAGCACC AGCTCAGCCA CACTGCACTG CCGTGCCCGG 2641 GGTGTCCCCA ACATCGACTT CACTTGGACC AAAAACGGGG TCCCTCTGGA TCTCCAAGAC 2701 CCCAGGTACA CAGAGCACAG GTACCACCAG GGTGTTGTCC ACAGCAGCCT CTTGACCATC 2761 GCTAATGTGT CTGCGGCCCA GGACTATGCC CTCTTCAAAT GCACGGCCAC CAATGCCCTT 2821 GGCTCTGACC ACACCAACAT CCAGCTCGTC AGCATCAGCC GCCCTGACCC TCCACTGGGA 2881 CTGAAGGTTG TCAGCATAAG CCCTCACTCG GTGGGGCTGG AGTGGAAGCC TGGCTTTGAT 2941 GGGGGTCTGC CTCAGAGGTT CCAAATCAGG TACGAGGCCC TCGAGACCCC AGGATTCCTC 3001 CACGTGGATG TCCTACCTAC ACAGGCCACT ACCTTCACGC TGACTGGGCT GAAGCCTTCT 3061 ACACGATATA GGATCTGGCT GTTGGCCAGC AATGCCCTGG GGGACAGTGG ATTGACGGAC 3121 AAGGGGATCC AGGTCTCCGT CACTACCCCA GGCCCCGACC AGGCTCCTGA AGACACAGAC 3181 CACCAGCTGC CCACAGAGCT GCCTCCAGGA CCCCCAAGGC TGCCCCTGCT GCCTGTGCTC 3241 TTTGCAGTTG GTGGTCTTCT GCTGCTCTCC AATGCCTCCT GTGTTGGGGG TCTCCTCTGG 3301 CGGAGAAGAC TGAGGCGCCT TGCTGAGGAG ATCTCAGAGA AGACAGAGGC AGGGTCGGAG 3361 GACAGGATCA GGAATGAATA TGAGGAGAGT CAGTGGACTG GGGACCGGGA CACGAGAAGC 3421 TCCACGGTTA GCACAGCAGA AGTGGACCCA AATTACTACT CCATGAGGGA CTTCAGCCCC 3481 CAGCTTCCCC CAACACTGGA GGAGGTGCTG TATCACCAAG GTGCTGAAGG CGAGGACATG 3541 GCCTTCCCCG GACACCTGCA TGATGAAGTG GAGAGAGCCT ATGGCCCGCC TGGGGCCTGG 3601 GGACCCCTCT ATGATGAAGT ACGAATGGAC CCCTATGACC TTCGCTGGCC TGAGGTCCAG 3661 TGTGAGGATC CGAGGGGAAT CTACGACCAG GTGGCAGCAG ACATGGATGC TGTGGAAGCT 3721 AGCTCTCTGC CGTTTGAGCT GAGGGGACAT CTGGTGTGA
Quality Control:cDNA sequencing results is available upon request.
