Rat NTRK2 Gene cDNA Clone / ORF Clone

NTRK2 cDNA Clone Datasheet

NTRK2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001163168.1
• cDNA Size: 1431
• Gene Synonym: Tkrb; trkB; TRKB1; RATTRKB1; Ntrk2
• Source: Rat

NTRK2 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus neurotrophic tyrosine kinase, receptor, type 2 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 816 T/C and 1302 C/T not causing the amino acid variation.

NTRK2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NTRK2 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NTRK2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCGCCCT GGCCGAGGTG GCATGGACCC GCCATGGCGC GGCTCTGGGG CTTATGCTTG
61       CTGGTCTTGG GCTTCTGGAG GGCTTCTCTT GCCTGCCCCA TGTCCTGCAA ATGCAGCACC
121      ACTAGGATTT GGTGTACCGA GCCTTCTCCT GGCATCGTGG CATTTCCGAG GTTGGAACCT
181      AACAGCATTG ACCCAGAGAA CATCACCGAA ATTCTCATTG CAAACCAGAA AAGGTTAGAA
241      ATCATCAATG AAGATGATGT CGAAGCTTAC GTGGGGCTGA AAAACCTTAC AATTGTGGAT
301      TCCGGCTTAA AGTTTGTGGC TTACAAGGCG TTTCTGAAGA ACGGCAACCT GCGGCACATC
361      AATTTCACTC GAAACAAGCT GACGAGTTTG TCCAGGAGAC ATTTCCGCCA CCTTGACTTG
421      TCTGACCTGA TCCTGACGGG TAATCCGTTC ACGTGTTCCT GTGACATCAT GTGGCTCAAG
481      ACTCTCCAGG AGACGAAATC CAGCCCCGAC ACTCAGGATT TGTATTGCCT CAATGAGAGC
541      AGCAAGAATA CCCCTCTGGC GAACCTGCAG ATTCCCAATT GTGGTCTGCC GTCTGCACGT
601      CTGGCCGCTC CTAACCTCAC GGTGGAGGAA GGGAAGTCTG TGACCATTTC CTGCAGCGTC
661      GGGGGTGACC CGCTCCCCAC CTTGTACTGG GACGTTGGGA ATTTGGTTTC CAAACACATG
721      AATGAAACAA GCCACACACA GGGCTCCTTA AGGATAACAA ACATTTCATC GGATGACAGT
781      GGGAAACAAA TCTCTTGTGT GGCAGAAAAC CTCGTCGGAG AAGATCAAGA CTCTGTGAAC
841      CTCACTGTGC ATTTTGCACC AACCATCACA TTTCTCGAAT CTCCAACCTC AGACCACCAC
901      TGGTGCATCC CATTCACTGT GAGAGGCAAC CCCAAGCCAG CACTTCAGTG GTTCTACAAC
961      GGAGCCATAC TGAATGAATC CAAGTACATC TGTACCAAAA TACACGTCAC CAATCACACG
1021     GAGTACCACG GCTGCCTCCA GCTGGATAAC CCCACTCATA TGAATAATGG AGACTACACC
1081     CTAATGGCCA AGAATGAATA TGGGAAGGAC GAGAGACAGA TTTCTGCTCA CTTCATGGGC
1141     CGGCCTGGAG TTGACTATGA GACAAACCCA AATTACCCTG AAGTCCTCTA TGAAGACTGG
1201     ACCACGCCAA CTGACATCGG GGATACTACA AACAAAAGTA ATGAGATCCC CTCCACGGAT
1261     GTTGCTGACC AAACCAATCG GGAGCATCTC TCGGTCTATG CTGTGGTGGT GATTGCCTCT
1321     GTGGTAGGAT TCTGCCTGCT GGTGATGCTG CTTCTGCTCA AGTTGGCGAG ACATTCCAAG
1381     TTTGGCATGA AAGGTTTTGT TTTGTTTCAT AAGATCCCCC TGGATGGGTA G

 

Quality Control:cDNA sequencing results is available upon request.

TrkB cDNA Clone Related Products &Topics

Related Areas:

Enzyme>>Protein Kinase>>Receptor Tyrosine Kinase>>TrkB/NTRK2

Neuroscience>>Neurotrophic Factor & Receptor>>Neurotrophin & Receptor>>TrkB/NTRK2

Signal Transduction>>Protein Kinase>>Receptor Tyrosine Kinase>>TrkB/NTRK2

Cancer>>Growth Factor & Receptor>> Neurotrophin & Receptor>>TrkB/NTRK2

Cancer>>Growth Factor & Receptor>>Receptor Tyrosine Kinase>>TrkB/NTRK2

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
TrkB/NTRK2	Human	TrkB/NTRK2/Fc Protein, Recombinant	10047-H03H
TrkB/NTRK2	Human	TrkB/NTRK2 Protein, Recombinant	10047-H08H
TrkB/NTRK2	Mouse	TrkB/NTRK2 Protein, Recombinant	50132-M08H
TrkB/NTRK2	Rat	NTRK2 / TrkB Protein, Recombinant	80243-R02H
TrkB/NTRK2	Rat	NTRK2 / TrkB Protein, Recombinant	80243-R08H

Antibodies:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human TrkB/NTRK2	WB	Mouse Monoclonal Antibody	10047-MM05
Human TrkB/NTRK2	ELISA	NTRK2 / Trk-B / TRKB Antibody	10047-MM12
Human TrkB/NTRK2	WB, ELISA, IHC	TrkB/NTRK2 Antibody, Rabbit MAb	10047-R107
Human TrkB/NTRK2	WB, ELISA	Rabbit Polyclonal Antibody	10047-RP01
Human TrkB/NTRK2	WB, ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	10047-RP02

ELISA Kits:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human TrkB/NTRK2	ELISA	TrkB/NTRK2 ELISA Kit	SEK10047

cDNA Clones:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
TrkB/NTRK2	Mouse	Mus musculus TrkB/NTRK2 cDNA Clone	MG50132-M
TrkB/NTRK2	Canine	Canine NTRK2 Gene cDNA Clone / ORF Clone	DG70035-G
TrkB/NTRK2	Rat	Rat NTRK2 cDNA Clone / ORF Clone	RG80243-G

TrkB Gene Description

TrkB is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor exclusively expressed in neurons of the central nervous system. BDNF(brain-derived neurotrophic factor) exerts biological actions through its physiological receptor TrkB which leads to the initiation of intracellular signaling cascades and different biological responses in neurons. TRKB has two distinct binding regions for BDNF within the extracellular domain with high affinity. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. Upon neurotrophin binding, TrkB phosphorylates itself and the downstream molecular members of the related signal pathways. TrkB has been shown to play an important role in tumorigenesis and metastasis, and mutations in this gene are associated with obesity and mood disorders. Accordingly, BDNF/TrkB signaling is thought to be a potential therapeutic target for schizophrenia.

References

1. Klein, R. et al., 1990, Cell. 64: 647-656.
2. Haniu, M. et al., 1997, J. Biol. Chem. 272 : 25296-25303.
3. Haapasalo, A. et al., 2002, J. Biol. Chem. 277: 43160-43167.
4. Pillai, A., 2008, Neurosignals. 16: 183-193.
5. Thomas, R. et al., 2007, Cancer. Res. 67: 6221-6229.