Rat NTRK2 / TrkB Protein (His Tag)

Neurotrophic tyrosine kinase, receptor, type 2 Protein Datasheet

NTRK2 / TrkB Protein Product Information

• Synonym: NTRK2, Trkb
• Protein Construction: A DNA sequence encoding the rat NTRK2 (Q63604-Isoform T1)(Met1-His429) was expressed with a polyhistidine tag at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

NTRK2 / TrkB Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Cys 32
• Molecular Mass: The recombinant rat NTRK2 comprises 409 amino acids and predicts a molecular mass of 47.1 kDa. The apparent molecular mass of the recombinant protein is approximately 67 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: NTRK2 / TrkB protein

NTRK2 / TrkB Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

NTRK2 / TrkB Protein Description

TrkB is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor exclusively expressed in neurons of the central nervous system. BDNF( brain-derived neurotrophic factor ) exerts biological actions through its physiological receptor TrkB which leads to the initiation of intracellular signaling cascades and different biological responses in neurons. TRKB has two distinct binding regions for BDNF within the extracellular domain with high affinity. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. Upon neurotrophin binding, TrkB phosphorylates itself and the downstream molecular members of the related signal pathways. TrkB has been shown to play an important role in tumorigenesis and metastasis, and mutations in this gene are associated with obesity and mood disorders. Accordingly, BDNF/TrkB signaling is thought to be a potential therapeutic target for schizophrenia.

References

1. Klein R. et al., 1990, Cell. 64: 647-56.
2. Haniu M. et al., 1997, J Biol Chem. 272: 25296-303.
3. Haapasalo A. et al., 2002, J Biol Chem. 277: 43160-7.
4. Pillai A. 2008, Neurosignals. 16: 183-93.
5. Mas R. et al., 2007, Cancer Res. 67: 6221-9.