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Mouse NTRK1 Gene cDNA Clone (full-length ORF Clone)
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Mouse NTRK1 Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
MG51103-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

Antibody Production Services

NTRK1 cDNA Clone Datasheet

 

NTRK1 cDNA Clone Product Information

Gene Bank Ref.ID: NM_001033124.1

cDNA Size:

2400

Gene Synonym:

Tkr; trk; TrkA; C80751; Ntrk1

Source: Mouse

NTRK1 cDNA Clone Description

cDNA Description:

ORF Clone of Mus musculus neurotrophic tyrosine kinase, receptor, type 1 DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1276 G/A not causing the amino acid variation.

NTRK1 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NTRK1 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NTRK1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGCGAG GCCAGCGGCT CGGGCAGCTG GGCTGGCATC GCCCGGCCGC GGGGCTAGGC
61       AGTCTGATGA CTTCGTTGAT GCTGGCCTGT GCCTCCGCCG CATCCTGTCG CGAGGTCTGC
121      TGTCCCGTGG GCCCCTCGGG GCTGCGCTGC ACCAGGGCAG GGTCCCTGGA TACCCTCCGC
181      GGCCTGCGGG GCGCCGGGAA CCTGACGGAG CTCTACGTGG AAAACCAGCA GCACCTGCAA
241      CGCTTGGAGT TTGAGGACCT GCAGGGCCTG GGGGAGTTGA GAAGCCTAAC CATCGTGAAG
301      AGTGGCCTCC GCTTTGTGGC CCCAGATGCC TTCCGTTTCA CCCCTCGGCT CAGTCACCTG
361      AATCTGTCCT CCAATGCGTT GGAGTCCCTC TCCTGGAAAA CTGTGCAGGG CCTCTCCCTA
421      CAGGACCTGA CCCTGTCAGG GAACCCACTG CATTGTTCCT GTGCCCTGTT CTGGCTCCAG
481      CGTTGGGAGC AGGAAGGGCT GTGTGGTGTG CATACACAGA CGCTTCATGA CTCTGGGCCT
541      GGAGACCAGT TCCTCCCACT GGGACACAAC ACCAGTTGTG GTGTACCCAC AGTGAAGATC
601      CAGATGCCCA ATGACTCTGT GGAAGTGGGC GATGACGTGT TTCTGCAGTG CCAGGTGGAG
661      GGGCTGGCCC TACAGCAGGC TGACTGGATC CTCACAGAGC TGGAAGGGGC AGCCACCGTG
721      AAGAAATTTG GAGATCTGCC ATCCCTGGGG CTGATTCTGG TCAATGTCAC CAGTGATCTC
781      AACAAGAAGA ATGTGACGTG CTGGGCAGAG AATGATGTGG GCCGGGCCGA GGTCTCTGTC
841      CAAGTCAGCG TCTCCTTCCC AGCCAGTGTG CACCTGGGCC TAGCGGTGGA GCAGCATCAT
901      TGGTGCATCC CCTTCTCGGT GGACGGGCAG CCAGCACCGT CTCTGCGCTG GTTGTTCAAC
961      GGCTCTGTGC TCAACGAGAC CAGTTTCATC TTCACTCAGT TCTTGGAGTC TGCGCTGACT
1021     AATGAGACCA TGCGGCACGG CTGCCTGCGC CTCAATCAGC CCACGCATGT CAACAACGGG
1081     AACTACACCC TGCTGGCGGC CAACCCCTAC GGCCAGGCTG CCGCCTCCGT CATGGCTGCT
1141     TTTATGGACA ACCCTTTTGA GTTCAACCCT GAGGACCCCA TCCCTGTCTC CTTCTCGCCA
1201     GTGGACGGTA ACAGCACATC AAGAGACCCA GTGGAGAAGA AAGATGAAAC CCCTTTTGGG
1261     GTCTCTGTGG CTGTGAGCCT AGCCGTCTCT GCCGCCCTCT TCCTTTCTGC CCTCCTTCTT
1321     GTGCTCAACA AATGTGGACA GAGGAGCAAA TTTGGGATCA ACCGCCCTGC TGTATTAGCT
1381     CCAGAGGATG GGCTGGCCAT GTCCCTACAC TTCATGACAC TGGGTGGCAG TTCTCTTTCC
1441     CCTACTGAGG GCAAAGGCTC CGGACTCCAG GGCCACATCA TGGAGAACCC ACAGTACTTC
1501     AGTGATACCT GTGTCCATCA CATCAAGCGC CAGGACATCA TTCTCAAGTG GGAGCTAGGG
1561     GAGGGAGCCT TTGGAAAGGT CTTTCTCGCT GAGTGCTACA ACCTTCTGAA TGATCAGGAC
1621     AAGATGCTCG TGGCTGTCAA GGCACTGAAG GAGGCATCCG AGAATGCTCG GCAGGACTTT
1681     CAGCGTGAGG CCGAGCTGCT CACCATGCTA CAGCACCAGC ACATCGTGCG CTTCTTTGGA
1741     GTCTGCACCG AGGGTGGCCC GTTGCTCATG GTCTTTGAGT ACATGCGCCA CGGGGACCTC
1801     AACCGTTTCC TCCGGTCCCA CGGACCTGAT GCAAAGCTGC TGGCTGGTGG TGAGGATGTG
1861     GCTCCTGGTC CTCTGGGCCT TGGGCAGCTT CTGGCTGTGG CTAGCCAGGT GGCTGCTGGT
1921     ATGGTATATC TAGCCAGCCT GCACTTTGTG CACCGGGATC TGGCCACACG CAACTGTCTG
1981     GTGGGTCAGG GACTAGTGGT GAAGATTGGA GACTTTGGCA TGAGCAGAGA CATCTATAGC
2041     ACAGACTATT ACCGAGTGGG AGGTCGGACC ATGCTGCCCA TCCGCTGGAT GCCACCCGAG
2101     AGCATCCTCT ACCGCAAGTT CAGCACCGAG AGCGATGTGT GGAGCTTTGG GGTGGTGCTC
2161     TGGGAGATCT TCACCTATGG AAAGCAGCCC TGGTACCAGC TCTCCAACAC TGAGGCGATC
2221     GAGTGTATCA CGCAGGGCCG GGAGCTGGAG CGGCCGCGCG CCTGCCCTCC TGATGTCTAC
2281     GCCATCATGC GAGGCTGCTG GCAGCGAGAA CCGCAGCAAC GCCTCAGCAT GAAGGATGTG
2341     CACGCGCGTC TGCAGGCCCT GGCACAGGCG CCACCCAGTT ACCTGGACGT TCTGGGCTAG

 

Quality Control:cDNA sequencing results is available upon request.

TrkA cDNA Clone Related Products & Topics

TrkA Protein Related Areas:

Enzyme>>Protein Kinase>>Receptor Tyrosine Kinase>>TrkA/NTRK1

Neuroscience>>Neurotrophic Factor & Receptor>>Neurotrophin & Receptor>>TrkA/NTRK1

Signal Transduction>>Protein Kinase>>Receptor Tyrosine Kinase>>TrkA/NTRK1

Cancer>>Growth Factor & Receptor>> Neurotrophin & Receptor>>TrkA/NTRK1

Cancer>>Growth Factor & Receptor>>Receptor Tyrosine Kinase>>TrkA/NTRK1

Proteins:

Molecule Species Description //For Detailed Info. and Price------CLICK! Cat. No
TrkA/NTRK1 Human TrkA/NTRK1/Fc Protein, Recombinant 11073-H03H
TrkA/NTRK1 Human TrkA/NTRK1 Protein (aa 194-413), Recombinant 11073-H07H2
TrkA/NTRK1 Human TrkA/NTRK1 Protein (aa 285-413), Recombinant 11073-H07E1
TrkA/NTRK1 Human TrkA/NTRK1 Protein, Recombinant 11073-H08H

Antibodies:

Molecule Application Description //For Detailed Info. and Price------CLICK! Cat. No
Human
TrkA/NTRK1
WB, ELISA Mouse Monoclonal Antibody 11073-MM04
Human
TrkA/NTRK1
WB, ELISA Mouse Monoclonal Antibody 11073-MM09
Human
TrkA/NTRK1
WB, ELISA TrkA/NTRK1 Antibody, Rabbit MAb 11073-R001
Human
TrkA/NTRK1
IHC-P TrkA / NTRK1 Antibody 11073-R024
Human
TrkA/NTRK1
WB, ELISA Rabbit Polyclonal Antibody 11073-RP01
Human
TrkA/NTRK1
WB, ELISA Rabbit Polyclonal Antibody (Antigen Affinity Purified) 11073-RP02

ELISA Kits:

Molecule Application Description //For Detailed Info. and Price------CLICK! Cat. No
Human TrkA/NTRK1 ELISA TrkA/NTRK1 ELISA Kit SEK11073

cDNA Clones:

Molecule Species Description //For Detailed Info. and Price------CLICK! Cat. No
TrkA/NTRK1 Human Homo sapiens TrkA/NTRK1 cDNA Clone HG11073-M
TrkA/NTRK1 Rat NTRK1 cDNA Clone / ORF Clone RG80404-G

TrkA Gene Description

Neurotrophic tyrosine kinase, receptor, type 1 (NTRK1), also well known as TrkA, is a member of the neurotrophic tyrosine kinase receptor family belonging to Tyr protein kinase superfamily, which also includes TrkB and TrkC. TrkA/NTRK1 has a complex subdomain orgnazation conserved in the family members, containing two cysteine-rich domains, a cluster of three leucine-rich motifs (LRR), and two Ig-like C2-type domains in the extracellular region, as well as an tyrosine kinase domain in the cytoplasmic region. TrkA/NTRK1 exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures, and is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA/NTRK1 plays an important role in cell survival, differentiation, and apoptosis in various neuronal and nonneuronal cell types, especially in the nervous system by recruiting signaling molecules including SHC1, PI 3-kinase, and PLC-gamma-1 that activate pathways required for the growth and survival of neurons. It has been shown that TrkA/NTRK1 is specially involved in the development and function of the nociceptive reception system and establishment of thermal regulation via sweating. Two distinct TrkA/NTRK1 isoforms have been identified: TrkA-II is primarily expressed in neuronal cells, while isoform TrkA-I is found in non-neuronal tissues. Defects in TrkA/NTRK1 are a cause of a rare autosomal recessive disorder, congenital insensitivity to pain with anhidrosis (CIPA).

References

  1. Bossemeyer. D. et al., 1989, J. Biol. Chem. 264:16403-16410.
  2. Varsano,T. et al., 2006 , Mol. Cell. Biol. 26: 8942–8952.
  3. Perrone, L. et al., 2005, Biochem J. 385: 233–241.
  4. Holtzman,D.M. et al., 2009 , J. Neurosci. 15: 1567–1576.
  5. Dan C. Lin et al., 2006,Mol Cell Biol. 26: 8928–8941.
  6. Jung, E.J. et al., 2008, Exp. Mol. Med. 40: 276–285.

Neurotrophic Factors & Receptors Related Information

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"