Mouse NTRK1 Gene cDNA Clone / ORF Clone

NTRK1 cDNA Clone Datasheet

NTRK1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001033124.1
• cDNA Size: 2400
• Gene Synonym: Tkr; trk; TrkA; C80751; Ntrk1
• Source: Mouse

NTRK1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus neurotrophic tyrosine kinase, receptor, type 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1276 G/A not causing the amino acid variation.

NTRK1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NTRK1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NTRK1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGCGAG GCCAGCGGCT CGGGCAGCTG GGCTGGCATC GCCCGGCCGC GGGGCTAGGC
61       AGTCTGATGA CTTCGTTGAT GCTGGCCTGT GCCTCCGCCG CATCCTGTCG CGAGGTCTGC
121      TGTCCCGTGG GCCCCTCGGG GCTGCGCTGC ACCAGGGCAG GGTCCCTGGA TACCCTCCGC
181      GGCCTGCGGG GCGCCGGGAA CCTGACGGAG CTCTACGTGG AAAACCAGCA GCACCTGCAA
241      CGCTTGGAGT TTGAGGACCT GCAGGGCCTG GGGGAGTTGA GAAGCCTAAC CATCGTGAAG
301      AGTGGCCTCC GCTTTGTGGC CCCAGATGCC TTCCGTTTCA CCCCTCGGCT CAGTCACCTG
361      AATCTGTCCT CCAATGCGTT GGAGTCCCTC TCCTGGAAAA CTGTGCAGGG CCTCTCCCTA
421      CAGGACCTGA CCCTGTCAGG GAACCCACTG CATTGTTCCT GTGCCCTGTT CTGGCTCCAG
481      CGTTGGGAGC AGGAAGGGCT GTGTGGTGTG CATACACAGA CGCTTCATGA CTCTGGGCCT
541      GGAGACCAGT TCCTCCCACT GGGACACAAC ACCAGTTGTG GTGTACCCAC AGTGAAGATC
601      CAGATGCCCA ATGACTCTGT GGAAGTGGGC GATGACGTGT TTCTGCAGTG CCAGGTGGAG
661      GGGCTGGCCC TACAGCAGGC TGACTGGATC CTCACAGAGC TGGAAGGGGC AGCCACCGTG
721      AAGAAATTTG GAGATCTGCC ATCCCTGGGG CTGATTCTGG TCAATGTCAC CAGTGATCTC
781      AACAAGAAGA ATGTGACGTG CTGGGCAGAG AATGATGTGG GCCGGGCCGA GGTCTCTGTC
841      CAAGTCAGCG TCTCCTTCCC AGCCAGTGTG CACCTGGGCC TAGCGGTGGA GCAGCATCAT
901      TGGTGCATCC CCTTCTCGGT GGACGGGCAG CCAGCACCGT CTCTGCGCTG GTTGTTCAAC
961      GGCTCTGTGC TCAACGAGAC CAGTTTCATC TTCACTCAGT TCTTGGAGTC TGCGCTGACT
1021     AATGAGACCA TGCGGCACGG CTGCCTGCGC CTCAATCAGC CCACGCATGT CAACAACGGG
1081     AACTACACCC TGCTGGCGGC CAACCCCTAC GGCCAGGCTG CCGCCTCCGT CATGGCTGCT
1141     TTTATGGACA ACCCTTTTGA GTTCAACCCT GAGGACCCCA TCCCTGTCTC CTTCTCGCCA
1201     GTGGACGGTA ACAGCACATC AAGAGACCCA GTGGAGAAGA AAGATGAAAC CCCTTTTGGG
1261     GTCTCTGTGG CTGTGAGCCT AGCCGTCTCT GCCGCCCTCT TCCTTTCTGC CCTCCTTCTT
1321     GTGCTCAACA AATGTGGACA GAGGAGCAAA TTTGGGATCA ACCGCCCTGC TGTATTAGCT
1381     CCAGAGGATG GGCTGGCCAT GTCCCTACAC TTCATGACAC TGGGTGGCAG TTCTCTTTCC
1441     CCTACTGAGG GCAAAGGCTC CGGACTCCAG GGCCACATCA TGGAGAACCC ACAGTACTTC
1501     AGTGATACCT GTGTCCATCA CATCAAGCGC CAGGACATCA TTCTCAAGTG GGAGCTAGGG
1561     GAGGGAGCCT TTGGAAAGGT CTTTCTCGCT GAGTGCTACA ACCTTCTGAA TGATCAGGAC
1621     AAGATGCTCG TGGCTGTCAA GGCACTGAAG GAGGCATCCG AGAATGCTCG GCAGGACTTT
1681     CAGCGTGAGG CCGAGCTGCT CACCATGCTA CAGCACCAGC ACATCGTGCG CTTCTTTGGA
1741     GTCTGCACCG AGGGTGGCCC GTTGCTCATG GTCTTTGAGT ACATGCGCCA CGGGGACCTC
1801     AACCGTTTCC TCCGGTCCCA CGGACCTGAT GCAAAGCTGC TGGCTGGTGG TGAGGATGTG
1861     GCTCCTGGTC CTCTGGGCCT TGGGCAGCTT CTGGCTGTGG CTAGCCAGGT GGCTGCTGGT
1921     ATGGTATATC TAGCCAGCCT GCACTTTGTG CACCGGGATC TGGCCACACG CAACTGTCTG
1981     GTGGGTCAGG GACTAGTGGT GAAGATTGGA GACTTTGGCA TGAGCAGAGA CATCTATAGC
2041     ACAGACTATT ACCGAGTGGG AGGTCGGACC ATGCTGCCCA TCCGCTGGAT GCCACCCGAG
2101     AGCATCCTCT ACCGCAAGTT CAGCACCGAG AGCGATGTGT GGAGCTTTGG GGTGGTGCTC
2161     TGGGAGATCT TCACCTATGG AAAGCAGCCC TGGTACCAGC TCTCCAACAC TGAGGCGATC
2221     GAGTGTATCA CGCAGGGCCG GGAGCTGGAG CGGCCGCGCG CCTGCCCTCC TGATGTCTAC
2281     GCCATCATGC GAGGCTGCTG GCAGCGAGAA CCGCAGCAAC GCCTCAGCAT GAAGGATGTG
2341     CACGCGCGTC TGCAGGCCCT GGCACAGGCG CCACCCAGTT ACCTGGACGT TCTGGGCTAG

 

Quality Control:cDNA sequencing results is available upon request.

TrkA Gene Description

Neurotrophic tyrosine kinase, receptor, type 1 (NTRK1), also well known as TrkA, is a member of the neurotrophic tyrosine kinase receptor family belonging to Tyr protein kinase superfamily, which also includes TrkB and TrkC. TrkA/NTRK1 has a complex subdomain orgnazation conserved in the family members, containing two cysteine-rich domains, a cluster of three leucine-rich motifs (LRR), and two Ig-like C2-type domains in the extracellular region, as well as an tyrosine kinase domain in the cytoplasmic region. TrkA/NTRK1 exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures, and is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA/NTRK1 plays an important role in cell survival, differentiation, and apoptosis in various neuronal and nonneuronal cell types, especially in the nervous system by recruiting signaling molecules including SHC1, PI 3-kinase, and PLC-gamma-1 that activate pathways required for the growth and survival of neurons. It has been shown that TrkA/NTRK1 is specially involved in the development and function of the nociceptive reception system and establishment of thermal regulation via sweating. Two distinct TrkA/NTRK1 isoforms have been identified: TrkA-II is primarily expressed in neuronal cells, while isoform TrkA-I is found in non-neuronal tissues. Defects in TrkA/NTRK1 are a cause of a rare autosomal recessive disorder, congenital insensitivity to pain with anhidrosis (CIPA).