Rat NTRK1 Gene cDNA Clone / ORF Clone

NTRK1 cDNA Clone Datasheet

NTRK1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_021589.1
• cDNA Size: 2400
• Gene Synonym: Trk; Ntrk1
• Source: Rat

NTRK1 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus neurotrophic tyrosine kinase, receptor, type 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1493 A/G resulting in the amino acid Gln substitution by Arg and 1293 C/T not causing the amino acid variation.

NTRK1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NTRK1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NTRK1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGCGAG GCCAGCGGCA CGGGCAGCTG GGTTGGCATC GCCCGGCCGC GGGGCTAGGC
61       GGTCTGGTGA CTTCGTTGAT GCTGGCTTGT GCTTGCGCCG CATCCTGTCG TGAGACCTGC
121      TGTCCCGTGG GCCCCTCGGG GTTGCGCTGC ACCAGGGCAG GGACCCTGAA TACCCTCCGC
181      GGCCTGCGGG GCGCCGGGAA CCTGACGGAG CTCTATGTGG AAAACCAGCG GGATCTGCAA
241      CGCCTGGAGT TTGAGGACCT GCAGGGCCTG GGGGAGTTGA GAAGCCTAAC CATCGTGAAG
301      AGTGGCCTCC GCTTTGTGGC CCCTGATGCC TTCCATTTCA CCCCTCGGCT CAGTCACCTG
361      AATCTCTCCT CCAATGCTTT GGAGTCCCTC TCCTGGAAAA CTGTGCAGGG CCTCTCCCTA
421      CAGGACTTGA CCCTGTCAGG GAACCCACTG CACTGTTCCT GTGCCCTATT GTGGCTCCAG
481      CGCTGGGAGC AGGAGGATTT GTGTGGTGTG TATACACAAA AGCTTCAGGG CTCTGGGTCT
541      GGAGACCAGT TCCTCCCACT GGGACACAAC AACAGTTGTG GTGTACCCTC AGTGAAGATC
601      CAGATGCCCA ATGACTCTGT GGAAGTGGGG GATGACGTTT TTCTGCAGTG CCAGGTGGAG
661      GGGCAGGCCC TACAGCAGGC TGACTGGATC CTCACAGAGC TGGAAGGGAC AGCCACCATG
721      AAGAAATCTG GAGATCTGCC ATCCCTGGGG CTAACTCTGG TCAATGTCAC CAGTGATCTC
781      AACAAGAAGA ATGTGACGTG CTGGGCAGAG AATGATGTGG GCCGGGCTGA GGTCTCTGTC
841      CAAGTCAGCG TCTCCTTCCC AGCCAGTGTG CATCTGGGCA AAGCCGTGGA ACAGCATCAC
901      TGGTGCATTC CCTTCTCTGT GGACGGGCAG CCAGCACCGT CCCTGCGCTG GTTCTTCAAC
961      GGCTCTGTGC TCAATGAGAC CAGCTTCATC TTCACTCAGT TCTTGGAGTC AGCGCTGACC
1021     AATGAGACCA TGCGGCATGG CTGCCTTCGC CTCAACCAGC CCACGCATGT CAACAACGGG
1081     AACTACACCC TGCTGGCTGC CAACCCCTAT GGCCAGGCTG CTGCCTCCAT CATGGCTGCC
1141     TTTATGGACA ACCCTTTTGA GTTCAACCCT GAGGACCCCA TCCCTGTCTC CTTCTCGCCA
1201     GTGGACACTA ACAGCACATC AAGAGACCCA GTGGAGAAGA AGGACGAAAC ACCTTTTGGG
1261     GTCTCTGTGG CTGTGGGCCT GGCCGTCTCC GCTGCCCTCT TCCTTTCTGC CCTCCTCCTA
1321     GTGCTCAACA AATGTGGACA GAGGAGCAAA TTTGGGATCA ACCGCCCTGC TGTGCTGGCG
1381     CCAGAGGATG GGCTGGCCAT GTCCCTACAC TTCATGACAC TGGGTGGCAG TTCTCTTTCC
1441     CCTACTGAGG GCAAAGGCTC CGGACTCCAG GGCCACATCA TGGAGAACCC ACGGTACTTC
1501     AGTGATACCT GTGTCCACCA TATCAAGCGC CAGGACATCA TTCTCAAGTG GGAGCTAGGG
1561     GAGGGAGCCT TTGGAAAGGT CTTTCTTGCT GAGTGCTACA ACCTTCTGAA TGATCAGGAC
1621     AAGATGCTGG TGGCTGTCAA GGCACTGAAG GAGACATCTG AGAATGCTCG TCAGGACTTC
1681     CATCGTGAGG CAGAGCTGCT CACCATGCTA CAGCACCAAC ACATCGTACG CTTCTTTGGA
1741     GTCTGCACGG AGGGTGGCCC ATTGCTCATG GTCTTCGAGT ACATGCGCCA TGGGGACCTC
1801     AACCGTTTCC TCCGGTCCCA CGGACCTGAT GCAAAACTGC TGGCTGGCGG CGAGGATGTG
1861     GCTCCTGGTC CTTTGGGCCT TGGGCAGCTT CTGGCTGTGG CTAGCCAGGT GGCTGCTGGT
1921     ATGGTGTACC TAGCCAGCCT GCACTTTGTG CACCGGGACC TGGCCACACG CAACTGTCTG
1981     GTGGGTCAGG GACTAGTGGT CAAGATTGGA GACTTCGGCA TGAGCAGGGA CATCTACAGC
2041     ACAGACTACT ACCGTGTGGG AGGTCGGACC ATGCTGCCCA TCCGCTGGAT GCCTCCAGAG
2101     AGCATCCTCT ACCGCAAGTT CAGCACCGAG AGTGATGTGT GGAGCTTCGG GGTGGTGCTC
2161     TGGGAGATCT TCACCTATGG AAAGCAACCC TGGTACCAGC TCTCCAACAC TGAGGCGATC
2221     GAGTGCATCA CGCAGGGCCG GGAGCTGGAG CGGCCGCGCG CCTGCCCTCC TGATGTCTAC
2281     GCCATCATGC GCGGCTGCTG GCAGCGTGAG CCGCAACAGC GCCTCAGCAT GAAGGATGTG
2341     CACGCGCGGC TGCAGGCCTT GGCACAGGCG CCACCGAGTT ACCTGGACGT TCTGGGCTAG

 

Quality Control:cDNA sequencing results is available upon request.

TrkA Gene Description

Neurotrophic tyrosine kinase, receptor, type 1 (NTRK1), also well known as TrkA, is a member of the neurotrophic tyrosine kinase receptor family belonging to Tyr protein kinase superfamily, which also includes TrkB and TrkC. TrkA/NTRK1 has a complex subdomain orgnazation conserved in the family members, containing two cysteine-rich domains, a cluster of three leucine-rich motifs (LRR), and two Ig-like C2-type domains in the extracellular region, as well as an tyrosine kinase domain in the cytoplasmic region. TrkA/NTRK1 exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures, and is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA/NTRK1 plays an important role in cell survival, differentiation, and apoptosis in various neuronal and nonneuronal cell types, especially in the nervous system by recruiting signaling molecules including SHC1, PI 3-kinase, and PLC-gamma-1 that activate pathways required for the growth and survival of neurons. It has been shown that TrkA/NTRK1 is specially involved in the development and function of the nociceptive reception system and establishment of thermal regulation via sweating. Two distinct TrkA/NTRK1 isoforms have been identified: TrkA-II is primarily expressed in neuronal cells, while isoform TrkA-I is found in non-neuronal tissues. Defects in TrkA/NTRK1 are a cause of a rare autosomal recessive disorder, congenital insensitivity to pain with anhidrosis (CIPA).

References

1. Bossemeyer. D. et al., 1989, J. Biol. Chem. 264:16403-16410.
2. Varsano,T. et al., 2006 , Mol. Cell. Biol. 26: 8942–8952.
3. Perrone, L. et al., 2005, Biochem J. 385: 233–241.
4. Holtzman,D.M. et al., 2009 , J. Neurosci. 15: 1567–1576.
5. Dan C. Lin et al., 2006,Mol Cell Biol. 26: 8928–8941.
6. Jung, E.J. et al., 2008, Exp. Mol. Med. 40: 276–285.