Cynomolgus monkey NT5E Gene cDNA Clone / ORF Clone

NT5E cDNA Clone Datasheet

NT5E cDNA Clone Product Information

• cDNA Size: 1725
• Gene Synonym: NT5E
• Source: Cynomolgus monkey

NT5E cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) 5'-nucleotidase, ecto (CD73) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1337 C/T resulting in the amino acid Thr substitution by Ile and 1230 C/T, 1404 G/A not causing the amino acid variation.
  Please check the sequence information before order.

NT5E cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NT5E cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NT5E cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGTCCCC GAGCCGCGCG GGCGCCCGCG ACGCTACTCC TCGCCGTCGG CGCGCTGCTG
61       TGGTCTGCGG CCGGCGCCTG GGAGCTCACG ATTCTGCACA CCAACGACGT GCACAGCCGG
121      CTGGAGCAGA CCAGCGAGGA CTCCAGCAAG TGCGTCAACG CCAGCCGCTG CATGGGTGGT
181      GTGGCTCGGC TCTTCACGAA GGTGCAGCAG ATCCGACGCG CCGAACCCAA CGTGCTGCTG
241      CTGGACGCCG GCGACCAGTA CCAGGGCACC ATCTGGTTCA CCGTGTACAA GGGTGCCGAG
301      GTGGCGCACT TCATGAACGC CCTACGCTAC GATGCCATGG CACTGGGAAA TCATGAATTT
361      GATAATGGTG TAGAAGGACT GATTGAGCCA CTCCTCAAAG AGGCCAAATT TCCAATTCTG
421      AGTGCAAACA TTAAAGCAAA GGGGCCACTA GCATCTCAAA TATCAGGACT TTATTTGCCA
481      TATAAAGTTC TTCCTGTTGG TGATGAAGTT GTGGGAATCG TTGGATACAC TTCCAAAGAA
541      ACCCCTTTTC TCTCAAATCC AGGAACAAAT TTAGTGTTTG AAGATGAAAT CACTGCATTA
601      CAACCTGAAG TAGATAAGTT AAAAACTCTA AATGTGAACA AAATTATTGC ACTGGGACAT
661      TCGGGTTTTG AAACGGATAA ACTCATCGCT CAGAAAGTGA GGGGTGTGGA CGTCGTGGTG
721      GGAGGACACT CCAACACATT TCTTTACACA GGCAATCCAC CTTCCAAAGA GGTGCCTGCT
781      GGGAAGTACC CATTCATAGT CACTTCTGAT GATGGGCGGA AGGTTCCTGT AGTCCAGGCC
841      TATGCTTTTG GCAAATACCT AGGCTATCTG AAGATCGAGT TTGATGAAAG AGGAAACGTC
901      ATCTCTTCCC ATGGAAATCC CATTCTTCTA AACAGCAGCA TTCCTGAAGA TCCAAGCATA
961      AAAGCAGACA TTAACAAATG GAGAATAAAA TTGGACAATT ATTCTACCCA GGAATTGGGG
1021     AAAACAATTG TCTATCTGGA TGGCTCCTCT CAATCGTGCC GCTTTAGAGA ATGCAACATG
1081     GGCAACCTGA TTTGTGATGC TATGATTAAC AACAATCTCA GACACGCTGA TGAAATGTTC
1141     TGGAACCATG TATCCATGTG CATTTTAAAT GGAGGTGGTA TCCGGTCGCC CATTGATGAG
1201     CGCAACAATG GCACAATTAC CTGGGAGAAT CTGGCTGCTG TATTGCCCTT TGGAGGTACA
1261     TTTGACCTGG TCCAGTTAAA AGGTTCCACC CTGAAGAAGG CCTTTGAGCA CAGCGTGCAC
1321     CGCTATGGCC AGTCCATTGG AGAGTTCCTG CAGGTGGGCG GAATCCATGT GGTATATGAT
1381     CTTTCCCGAA AACCTGGAGA CAGAGTAGTC AAATTAGATG TTCTTTGCAC CAAGTGTCGA
1441     GTGCCCAGTT ATGACCCTCT CAAAATGGAT GAGATATATA AGGTGATCCT CCCAAACTTC
1501     CTGGCCAATG GTGGAGATGG ATTCCAGATG ATAAAAGATG AATTATTAAG ACATGACTCT
1561     GGTGATCAAG ATATCAATGT GGTTTCTACA TATATCTCAA AAATGAAAGT AATTTATCCA
1621     GCAGTTGAAG GTCGGATCAA GTTTTCCACA GGAAGTCATT GCCATGGAAG CTTTTCTTTA
1681     ATATTTCTTT CATTTTGTGC AGTGATCTTT GTTTTATACC AATAG

 

Quality Control:cDNA sequencing results is available upon request.