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Rat NT5E Gene cDNA Clone (full-length ORF Clone)
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Rat NT5E Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
RG80335-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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NT5E cDNA Clone Datasheet

 

NT5E cDNA Clone Product Information

Gene Bank Ref.ID: NM_021576.2

cDNA Size:

1731

Gene Synonym:

Nt5; CD73; MGC112615; Nt5e

Source: Rat

NT5E cDNA Clone Description

cDNA Description:

ORF Clone of Rattus norvegicus 5' nucleotidase, ecto DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

NT5E cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NT5E cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NT5E cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGTCCCG CGGCTGCTAC GGCACCCAAG TGGCTGCTTC TCGCGCTAAG CGCTCTACTA
61       CCACTGTGGC CCACAGCCAA ATCCTGGGAG CTCACGATCC TGCACACAAA CGACGTGCAC
121      AGCCGGCTAG AGCAAACCAG CGATGACTCC ACCAAGTGCC TCAACGCAAG CCTGTGTGTG
181      GGCGGCGTGG CCCGGCTCTT CACCAAGGTG CAGCAGATCC GCAAGGAAGA ACCCAACGTA
241      CTGCTTTTGG ATGCTGGCGA TCAGTACCAG GGCACCATCT GGTTCACTGT TTACAAAGGC
301      CTGGAAGTGG CACACTTCAT GAACCTCCTG GGCTACGATG CCATGGCACT GGGAAATCAT
361      GAATTTGATA ACGGTGTGGA AGGACTGATT GATCCCCTCC TCAGAAATGT CAAATTTCCA
421      ATTCTGAGTG CAAATATTAA GGCCCGGGGG CCACTAGCAC CTCAGATATC CGGACTTTAT
481      TTGCCATATA AAGTTCTCTC TGTCGGTGGT GAGGTTGTGG GGATTGTTGG ATATACTTCA
541      AAGGAAACCC CCTTCCTCTC AAATCCAGGG ACAAATTTAG TCTTCGAAGA TGAAGTCACT
601      GCGTTGCAGC CTGAAGTGGA TAAACTAAAA ACTCTAAACG TGAACAAGAT CATTGCCCTG
661      GGACACTCTG GTTTCGAGAT GGATAAACTC ATTGCCCAGA AGGTGAGAGG CGTGGACGTC
721      GTGGTGGGAG GACACACCAA CACCTTTCTC TACACAGGAA ATCCACCTTC CAAAGAAGTG
781      CCTGCTGGGA AGTACCCATT CATAGTCACC TCTGACGATG GGCGAAAGGT TCCCGTGGTC
841      CAGGCCTATG CCTTTGGCAA ATACCTGGGC TATCTGAAGG TTGAGTTTGA TGATAAAGGA
901      AATGTTGTCA CTTCCTATGG AAATCCCATT CTTCTGAACA GCACCATTCG TGAAGATGCA
961      GCCATCAAAG CAGACATTAA CCAGTGGAGG ATAAAATTAG ATAATTATTC TACACAGGAA
1021     CTCGGGAGAA CCATCGTCTA CCTGAATGGC TCCGCTCAGG AGTGCCGCTT CAGAGAGTGC
1081     AACATGGGAA ACCTGATCTG TGATGCCATG ATTAACAACA ACCTCAGACA CCCAGATGAA
1141     ATGTTTTGGA ACCACGTGTC CATGTGCATT GTAAATGGAG GTGGCATCCG GTCCCCCATT
1201     GATGAGAGGA ACAATGGTAC CATCACCTGG GAGAACCTGG CTGCTGTGCT GCCCTTTGGA
1261     GGGACATTTG ACCTTGTCCA ATTGAAAGGG TCCACCCTGA AGAAGGCTTT TGAGCACAGT
1321     GTGCATCGAT ATGGCCAGTC CACAGGGGAG TTCCTGCAAG TGGGTGGAAT CCATGTGGTG
1381     TATGATATTT CCCGAAAGCC CTGGGACAGA GTGGTCCAAT TAAAAGTTCT CTGCACCAAG
1441     TGTCGAGTGC CCATCTATGA GCCTCTCGAA ATGGATAAAG TGTATAAAGT GGTCCTCCCA
1501     AGCTATCTGG TCAACGGTGG GGATGGATTC CAAATGATAA AAGATGAATT ATTAAAACAT
1561     GACTCTGGTG ACCAAGATAT CAGTGTGGTT TCTGAATACA TCTCAAAAAT GAAAGTAATT
1621     TACCCAGCAG TTGAAGGACG GATCAAGTTC TCTGCAGCAA GTCATTACCA AGGAAGCTTT
1681     CCTTTAATAA TTCTTTCCTT TTGGGCAGTG ATCCTTGTTT TGTACCAATA A

 

Quality Control:cDNA sequencing results is available upon request.

 

 

CD73 (NT5E) related areas, pathways, and other information

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"