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Rat NT5E Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| RG80335-G |
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YES |  |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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NT5E cDNA Clone Datasheet
NT5E cDNA Clone Product Information
| Gene Bank Ref.ID: | NM_021576.2 |
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cDNA Size: |
1731 |
| Gene Synonym: |
Nt5; CD73; MGC112615; Nt5e |
| Source: | Rat |
NT5E cDNA Clone Description
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cDNA Description: |
ORF Clone of Rattus norvegicus 5' nucleotidase, ecto DNA. |
| Vector: | Cloning Vector: pGEM-T Vector
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Identical with the Gene Bank Ref.ID sequence. |
NT5E cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
NT5E cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
NT5E cDNA Clone Sequence and Quality Control
Sequence:
1 ATGCGTCCCG CGGCTGCTAC GGCACCCAAG TGGCTGCTTC TCGCGCTAAG CGCTCTACTA 61 CCACTGTGGC CCACAGCCAA ATCCTGGGAG CTCACGATCC TGCACACAAA CGACGTGCAC 121 AGCCGGCTAG AGCAAACCAG CGATGACTCC ACCAAGTGCC TCAACGCAAG CCTGTGTGTG 181 GGCGGCGTGG CCCGGCTCTT CACCAAGGTG CAGCAGATCC GCAAGGAAGA ACCCAACGTA 241 CTGCTTTTGG ATGCTGGCGA TCAGTACCAG GGCACCATCT GGTTCACTGT TTACAAAGGC 301 CTGGAAGTGG CACACTTCAT GAACCTCCTG GGCTACGATG CCATGGCACT GGGAAATCAT 361 GAATTTGATA ACGGTGTGGA AGGACTGATT GATCCCCTCC TCAGAAATGT CAAATTTCCA 421 ATTCTGAGTG CAAATATTAA GGCCCGGGGG CCACTAGCAC CTCAGATATC CGGACTTTAT 481 TTGCCATATA AAGTTCTCTC TGTCGGTGGT GAGGTTGTGG GGATTGTTGG ATATACTTCA 541 AAGGAAACCC CCTTCCTCTC AAATCCAGGG ACAAATTTAG TCTTCGAAGA TGAAGTCACT 601 GCGTTGCAGC CTGAAGTGGA TAAACTAAAA ACTCTAAACG TGAACAAGAT CATTGCCCTG 661 GGACACTCTG GTTTCGAGAT GGATAAACTC ATTGCCCAGA AGGTGAGAGG CGTGGACGTC 721 GTGGTGGGAG GACACACCAA CACCTTTCTC TACACAGGAA ATCCACCTTC CAAAGAAGTG 781 CCTGCTGGGA AGTACCCATT CATAGTCACC TCTGACGATG GGCGAAAGGT TCCCGTGGTC 841 CAGGCCTATG CCTTTGGCAA ATACCTGGGC TATCTGAAGG TTGAGTTTGA TGATAAAGGA 901 AATGTTGTCA CTTCCTATGG AAATCCCATT CTTCTGAACA GCACCATTCG TGAAGATGCA 961 GCCATCAAAG CAGACATTAA CCAGTGGAGG ATAAAATTAG ATAATTATTC TACACAGGAA 1021 CTCGGGAGAA CCATCGTCTA CCTGAATGGC TCCGCTCAGG AGTGCCGCTT CAGAGAGTGC 1081 AACATGGGAA ACCTGATCTG TGATGCCATG ATTAACAACA ACCTCAGACA CCCAGATGAA 1141 ATGTTTTGGA ACCACGTGTC CATGTGCATT GTAAATGGAG GTGGCATCCG GTCCCCCATT 1201 GATGAGAGGA ACAATGGTAC CATCACCTGG GAGAACCTGG CTGCTGTGCT GCCCTTTGGA 1261 GGGACATTTG ACCTTGTCCA ATTGAAAGGG TCCACCCTGA AGAAGGCTTT TGAGCACAGT 1321 GTGCATCGAT ATGGCCAGTC CACAGGGGAG TTCCTGCAAG TGGGTGGAAT CCATGTGGTG 1381 TATGATATTT CCCGAAAGCC CTGGGACAGA GTGGTCCAAT TAAAAGTTCT CTGCACCAAG 1441 TGTCGAGTGC CCATCTATGA GCCTCTCGAA ATGGATAAAG TGTATAAAGT GGTCCTCCCA 1501 AGCTATCTGG TCAACGGTGG GGATGGATTC CAAATGATAA AAGATGAATT ATTAAAACAT 1561 GACTCTGGTG ACCAAGATAT CAGTGTGGTT TCTGAATACA TCTCAAAAAT GAAAGTAATT 1621 TACCCAGCAG TTGAAGGACG GATCAAGTTC TCTGCAGCAA GTCATTACCA AGGAAGCTTT 1681 CCTTTAATAA TTCTTTCCTT TTGGGCAGTG ATCCTTGTTT TGTACCAATA A
Quality Control:cDNA sequencing results is available upon request.
