NT5C3 Protein (Native)

5'-nucleotidase, cytosolic III Protein Datasheet

NT5C3 Protein Product Information

• Synonym: P5N1, UMPH1, NT5C3
• Protein Construction: A DNA sequence encoding the mature form of human NT5C3 (NP_001002010) (Met 12- Leu 297) was expressed.
• Source: Human
• Expression Host: E.coli

NT5C3 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 12
• Molecular Mass: The recombinant human NT5C3 consists of 286 amino acids and predicts a molecular mass of 32.7KDa. It migrates as an approximately 32 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: NT5C3 protein

NT5C3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

NT5C3 Protein Description

NT5C3 is a member of the pyrimidine 5'-nucleotidase(P5N) family. The pyrimidine 5'-nucleotidase (P5N) includes type I and type II isozymes, which catalyze the dephosphorylation of the pyrimidine 5'-monophosphates UMP and CMP to the corresponding nucleosides. NT5C3 gene encodes the type I P5N isozyme and a gene on chromosome 17 encodes the type II P5N isozyme. Both genes are members of the nucleotidase gene family. Mutations in this gene are the main cause of P5N deficiency, which results in an autosomal recessive hemolytic anemia. It was reported that this gene had two pseudogenes on chromosomes 4 and 7, respectively, but the pseudogene on chromosome 7 is not verified. NT5C3 functions both as nucleotidase and as phosphotransferase. Defects in NT5C3 are the cause of P5N deficiency which is an autosomal recessive condition causing hemolytic anemia characterized by marked basophilic stipplig and the accumulation of high concentrations of pyrimidine nucleotides within the erythrocyte.

References

1. Hillier LD. et al., 1997, Genome Res. 6 (9): 807-28.
2. Jordheim LP. et al., 2008, Drug Metab Dispos. 36 (12): 2419-23.
3. Aksoy P. et al., 2009, Pharmacogenet Genomics. 19 (8): 567-76.