NSE / ENO2 / Enolase 2 Protein (His Tag)

Enolase 2 (gamma, neuronal) Protein Datasheet

NSE / ENO2 / Enolase 2 Protein Product Information

• Synonym: NSE
• Protein Construction: A DNA sequence encoding the mature form of human ENO2 ( P09104 ) ( Met 1-Leu 434 ) was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: E.coli

NSE / ENO2 / Enolase 2 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant human ENO2 comprises 444 amino acids and has a calculated molecular mass of 48.6KDa. It migrates as an approximately 48 kDa band as predicted in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of PBS, pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: NSE / ENO2 / Enolase 2 protein

NSE / ENO2 / Enolase 2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

NSE / ENO2 / Enolase 2 Protein Description

Gamma enolase, also known as enolase 2 (ENO2) or neuron specific enolase (NSE), is a phosphopyruvate hydratase belonging to the enolase family. ENO2 is one of the three enolase isoenzymes found in mammals and mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. The alpha / gamma heterodimer and the gamma / gamma homodimer are expressed in neurons. This isoenzyme is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. ENO2 has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It can bind to cultured neocortical neurons and promotes cell survival in a calcium-dependent manner. Levels of ENO2 increase dramatically in cardiovascular accidents, cerebral trauma, brain tumors and Creutzfildt-Jacob disease.

References

1. Pechumer H. et al., 1994, Lab Invest. 69 (6): 743-9.
2. Craig SP. et al., 1991, Cytogenet Cell Genet. 54 (1-2): 71-3.
3. Muley T. et al., 2003, Clin Chem Lab Med. 41 (1): 95-103.