NRXN3 / Neurexin 3 Antibody (Antigen Affinity Purified)

NRXN3 / Neurexin 3 Antibody ( Antigen Affinity Purified ) Datasheet

NRXN3 / Neurexin 3 Antibody Product Information

• Immunogen: Recombinant Human NRXN3 / Neurexin 3 protein (Catalog#11843-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human NRXN3 / Neurexin 3 (rh NRXN3 / Neurexin 3; Catalog#11843-H08H; NP_620426.2; Met 1-Thr 357). NRXN3 / Neurexin 3Human NRXN3 / Neurexin 3 affinity chromatography.

NRXN3 / Neurexin 3 Antibody Usage Guide

• Specificity: Human NRXN3 / Neurexin 3
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human NRXN3 in WB. Using a DAB detection system, the detection limit for Human NRXN3 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human NRXN3. The detection limit for Human NRXN3 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

NRXN3 / Neurexin 3 Antibody Background

Neurexin-3-beta, also known as Neurexin III-beta and NRXN3, is a single-pass type I membrane protein which belongs to theneurexin family. It contains onelaminin G-like domain. It is a neuronal cell surface protein that may be involved in cell recognition and cell adhesion. Neurexins are a family of proteins that function in the vertebrate nervous system as cell adhesion molecules and receptors. They are encoded by several unlinked genes of which two, NRXN1 and NRXN3, are among the largest known human genes. Three of the genes (NRXN1, NRXN2, NRXN3) utilize two alternate promoters and include numerous alternatively spliced exons to generate thousands of distinct mRNA transcripts and protein isoforms. The majority of transcripts are produced from the upstream promoter and encode alpha-neurexin isoforms; a much smaller number of transcripts are produced from the downstream promoter and encode beta-neurexin isoforms. The alpha-neurexins contain EGF-like sequences and laminin G domains, and have been shown to interact with neurexophilins. The beta-neurexins lack EGF-like sequences and contain fewer laminin G domains than alpha-neurexins. NRXN3 have been linked to genetic predisposition towards a number of conditions such as alcohol or drug addiction, or obesity.

References

1. Occhi G. et al., 2002, Biochem. Biophys. Res. Commun. 298:151-5.
2. Rowen L, et al., 2002, Genomics. 79 (4): 587-97.
3. Olsen J.V. et al., 2006, Cell 127: 635-48.
4. Hishimoto A, et al., 2007, Human Molecular Genetics.16 (23): 2880-91.
5. Oppermann F.S. et al., 2009, Mol. Cell. Proteomics 8: 1751-64.