Human NRP1 transcript variant 3 Gene cDNA Clone / ORF Clone

NRP1 cDNA Clone Datasheet

NRP1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001024629.2
• cDNA Size: 1830
• Gene Synonym: NP1; NRP; BDCA4; CD304; VEGF165R; NRP1
• Source: Human

NRP1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens neuropilin 1, transcript variant 3 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

NRP1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NRP1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NRP1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGAGGG GGCTGCCGCT CCTCTGCGCC GTGCTCGCCC TCGTCCTCGC CCCGGCCGGC
61       GCTTTTCGCA ACGATAAATG TGGCGATACT ATAAAAATTG AAAGCCCCGG GTACCTTACA
121      TCTCCTGGTT ATCCTCATTC TTATCACCCA AGTGAAAAAT GCGAATGGCT GATTCAGGCT
181      CCGGACCCAT ACCAGAGAAT TATGATCAAC TTCAACCCTC ACTTCGATTT GGAGGACAGA
241      GACTGCAAGT ATGACTACGT GGAAGTCTTC GATGGAGAAA ATGAAAATGG ACATTTTAGG
301      GGAAAGTTCT GTGGAAAGAT AGCCCCTCCT CCTGTTGTGT CTTCAGGGCC ATTTCTTTTT
361      ATCAAATTTG TCTCTGACTA CGAAACACAT GGTGCAGGAT TTTCCATACG TTATGAAATT
421      TTCAAGAGAG GTCCTGAATG TTCCCAGAAC TACACAACAC CTAGTGGAGT GATAAAGTCC
481      CCCGGATTCC CTGAAAAATA TCCCAACAGC CTTGAATGCA CTTATATTGT CTTTGCGCCA
541      AAGATGTCAG AGATTATCCT GGAATTTGAA AGCTTTGACC TGGAGCCTGA CTCAAATCCT
601      CCAGGGGGGA TGTTCTGTCG CTACGACCGG CTAGAAATCT GGGATGGATT CCCTGATGTT
661      GGCCCTCACA TTGGGCGTTA CTGTGGACAG AAAACACCAG GTCGAATCCG ATCCTCATCG
721      GGCATTCTCT CCATGGTTTT TTACACCGAC AGCGCGATAG CAAAAGAAGG TTTCTCAGCA
781      AACTACAGTG TCTTGCAGAG CAGTGTCTCA GAAGATTTCA AATGTATGGA AGCTCTGGGC
841      ATGGAATCAG GAGAAATTCA TTCTGACCAG ATCACAGCTT CTTCCCAGTA TAGCACCAAC
901      TGGTCTGCAG AGCGCTCCCG CCTGAACTAC CCTGAGAATG GGTGGACTCC CGGAGAGGAT
961      TCCTACCGAG AGTGGATACA GGTAGACTTG GGCCTTCTGC GCTTTGTCAC GGCTGTCGGG
1021     ACACAGGGCG CCATTTCAAA AGAAACCAAG AAGAAATATT ATGTCAAGAC TTACAAGATC
1081     GACGTTAGCT CCAACGGGGA AGACTGGATC ACCATAAAAG AAGGAAACAA ACCTGTTCTC
1141     TTTCAGGGAA ACACCAACCC CACAGATGTT GTGGTTGCAG TATTCCCCAA ACCACTGATA
1201     ACTCGATTTG TCCGAATCAA GCCTGCAACT TGGGAAACTG GCATATCTAT GAGATTTGAA
1261     GTATACGGTT GCAAGATAAC AGATTATCCT TGCTCTGGAA TGTTGGGTAT GGTGTCTGGA
1321     CTTATTTCTG ACTCCCAGAT CACATCATCC AACCAAGGGG ACAGAAACTG GATGCCTGAA
1381     AACATCCGCC TGGTAACCAG TCGCTCTGGC TGGGCACTTC CACCCGCACC TCATTCCTAC
1441     ATCAATGAGT GGCTCCAAAT AGACCTGGGG GAGGAGAAGA TCGTGAGGGG CATCATCATT
1501     CAGGGTGGGA AGCACCGAGA GAACAAGGTG TTCATGAGGA AGTTCAAGAT CGGGTACAGC
1561     AACAACGGCT CGGACTGGAA GATGATCATG GATGACAGCA AACGCAAGGC GAAGTCTTTT
1621     GAGGGCAACA ACAACTATGA TACACCTGAG CTGCGGACTT TTCCAGCTCT CTCCACGCGA
1681     TTCATCAGGA TCTACCCCGA GAGAGCCACT CATGGCGGAC TGGGGCTCAG AATGGAGCTG
1741     CTGGGCTGTG AAGTGGAAGG TGGCACCACT GTGCTGGCCA CAGAAAAGCC CACGGTCATA
1801     GACAGCACCA TACAATCAGG TATCAAATAA

 

Quality Control:cDNA sequencing results is available upon request.