NRN1 / Neuritin 1 Protein (His Tag)

Neuritin 1 Protein Datasheet

NRN1 / neuritin 1 Protein Product Information

• Synonym: MGC44811, NRN, dJ380B8.2
• Protein Construction: A DNA sequence encoding the mature form of human NRN1 (Q9NPD7) （Met 1-Asn 115）was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

NRN1 / neuritin 1 Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 28
• Molecular Mass: The secreted recombinant human NRN1 consists of 98 amino acids and migrates as an approximately 11 kDa band as predicted in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: NRN1 / neuritin 1 protein

NRN1 / neuritin 1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

NRN1 / neuritin 1 Protein Description

Neuritin 1 (NRN1) is a member of neuritin family. Neuritin is a glycosylphoshatidylinositol- anchored protein induced by neural activity. It is expressed in postmitotic-differentiating neurons of the developing nervous system and a population of small-diameter neurons in the dorsal root ganglia and was anterogradely and retrogradely transported. Neuritin message is induced by neuronal activity and by the activity-regulated neurotrophins BDNF, nerve growth factor (NGF) and NT-3. Purified recombinant neuritin promotes neurite outgrowth and arborization in primary embryonic hippocampal and cortical cultures. Thus, neuritin is considered as a downstream effector of activity-induced neurite outgrowth. In clinical, neuritin levels in diabetes were reduced in both dorsal root ganglia and sciatic nerve of rats, and these deficits were reversed in vivo by treatment with NGF. This manipulation of neuritin levels in diabetes may provide a potential target for the therapeutic intervention in the management of neuropathy.

References

1. Karamoysoyli E. et al., 2008, Diabetes. 57 (1): 181-9.
2. Naeve GS. et al., 1997, Proc Natl Acad Sci. 94 (6): 2648-53.
3. Kojima N. et al., 2005, FEBS Lett. 579 (21): 4562-6.