NRG1 (aa 2-246) Protein

Neuregulin 1 Protein Datasheet

NRG1 Protein Product Information

• Synonym: NRG1, GGF, HGL, HRGA, NDF, SMDF
• Protein Construction: A DNA sequence encoding the human NRG1(Q02297--isoform6) (Ser2-Lys246) was expressed, fused with the Fc region of mouse IgG1 at the N-terminus.
• Source: Human
• Expression Host: Human Cells

NRG1 Protein QC Testing

• Purity: > 88 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Asp
• Molecular Mass: The recombinant human NRG1/mFc comprises 481 amino acids and has a predicted molecular mass of 53.4 kDa. The apparent molecular mass of the monomer is approximately 67 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS,pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: NRG1 protein

NRG1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

NRG1 Protein Description

Neuregulin 1 or NRG1 is one of four proteins in the neuregulin family that act on the EGFR family of receptors. This growth factor was originally identified as a 44-kD glycoprotein that interacts with the NEU / ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. NRG1 is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. Neuregulin 1 (NRG1) is essential for the development and function of multiple organ systems, and its dysregulation has been linked to diseases such as cancer and schizophrenia. NRG1 is a schizophrenia candidate gene and plays an important role in brain development and neural function. Schizophrenia is a complex disorder, with etiology likely due to epistasis.

References

1. Nicodemus KK. et al., 2010, Arch Gen Psychiatry. 67 (10): 991-1001.
2. Tan W. et al., 2007, J Biol Chem. 282 (33): 24343-51.
3. Holmes WE. et al., 1992, Science. 256 (5060): 1205-10.