Mouse NRBP1 Gene cDNA Clone / ORF Clone

NRBP1 cDNA Clone Datasheet

NRBP1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_147201.2
• cDNA Size: 1608
• Gene Synonym: Nrbp; MGC6961; B230344L17Rik; Nrbp1
• Source: Mouse

NRBP1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus nuclear receptor binding protein 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 951 A/G and 1146 C/T not causing the amino acid variation.

NRBP1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NRBP1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NRBP1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCGGAAG GGGAGTCCCA AACAGTAGTC AGCAGTGGCT CAGACCCAAA GGTAGAATCC
61       TCATCTTTAG CTCCTGGCCT GACTTCTGTG TCACCTCCTG TGACCTCTAC AACTTCAGCT
121      GCTTCTCCCG AGGAAGAAGA AGAAAGTGAA GATGAGTCTG AGATCTTGGA AGAGTCACCC
181      TGTGGGCGCT GGCAGAAGAG GCGAGAAGAG GTGAATCAGC GTAATGTACC AGGCATTGAC
241      AGTGCATACC TGGCCATGGA TACAGAGGAA GGTGTGGAGG TTGTGTGGAA TGAGGTACAG
301      TTCTCTGAAC GCAAGAACTA CAAACTGCAG GAGGAAAAGG TCCGTGCAGT GTTTGATAAT
361      CTGATTCAGC TGGAACATCT TAACATTGTT AAGTTTCACA AATATTGGGC TGATGTTAAA
421      GAGAACAAGG CTAGGGTGAT TTTCATCACA GAATACATGT CCTCTGGGAG TCTTAAGCAG
481      TTTCTGAAGA AGACCAAAAA GAACCACAAG ACTATGAATG AAAAGGCTTG GAAACGCTGG
541      TGTACACAGA TCCTCTCTGC CCTAAGCTAC CTGCACTCCT GTGACCCTCC CATCATCCAT
601      GGGAACCTGA CCTGTGACAC CATCTTCATC CAGCACAACG GACTCATCAA GATTGGCTCT
661      GTGGCTCCTG ACACTATCAA CAATCACGTG AAGACTTGCC GGGAAGAACA GAAGAACCTA
721      CACTTTTTTG CACCAGAGTA TGGAGAAGTC ACAAACGTGA CAACAGCAGT GGACATCTAC
781      TCCTTTGGCA TGTGTGCACT GGAGATGGCA GTGCTGGAGA TTCAGGGCAA TGGCGAGTCC
841      TCATATGTGC CACAGGAAGC CATCAGCAGT GCCATCCAGC TACTAGAAGA CTCATTACAG
901      AGGGAGTTTA TTCAAAAGTG CCTGCAGTCT GAGCCTGCTC GGAGACCAAC GGCCAGAGAA
961      CTTCTGTTCC ACCCAGCACT GTTTGAAGTG CCCTCACTCA AGCTTCTTGC TGCTCACTGT
1021     ATCGTGGGGC ACCAACACAT GATCCCAGAG AACGCTCTAG AGGAGATCAC CAAGAACATG
1081     GATACCAGTG CTGTACTAGC TGAAATTCCC GCAGGGCCAG GACGAGAACC AGTTCAGACT
1141     TTGTATTCTC AGTCACCAGC CCTAGAATTA GACAAATTCC TTGAAGATGT CAGGAATGGG
1201     ATCTACCCTC TGACAGCCTT TGGGCTACCT CGGCCTCAGC AGCCACAGCA GGAGGAGGTG
1261     ACATCACCTG TTGTGCCCCC CTCTGTCAAG ACTCCAACTC CTGAGCCAGC TGAAGTGGAG
1321     ACACGAAAGG TGGTGCTGAT GCAGTGCAAC ATCGAATCTG TGGAGGAGGG AGTCAAACAC
1381     CATCTAACAC TTCTGCTGAA GCTGGAGGAC AAATTGAACC GGCACCTGAG CTGTGACCTG
1441     ATGCCAAATG AGAGCATCCC GGACTTGGCA GCTGAGCTGG TGCAGCTGGG CTTCATTAGT
1501     GAGGCTGATC AGAGCCGCCT GACTTCTCTG CTGGAGGAGA CGCTCAACAA GTTCAACTTC
1561     ACCAGGAACA GTACACTCAA CACAGCCACT GTCACCGTCT CCTCGTAG

 

Quality Control:cDNA sequencing results is available upon request.