Human NPPB Gene cDNA Clone / ORF Clone

NPPB cDNA Clone Datasheet

NPPB cDNA Clone Product Information

• Gene Bank Ref.ID: BC025785
• cDNA Size: 405
• Gene Synonym: BNP;NPPB
• Source: Human

NPPB cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens natriuretic peptide precursor B DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

NPPB cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NPPB cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NPPB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATCCCC AGACAGCACC TTCCCGGGCG CTCCTGCTCC TGCTCTTCTT GCATCTGGCT
61       TTCCTGGGAG GTCGTTCCCA CCCGCTGGGC AGCCCCGGTT CAGCCTCGGA CTTGGAAACG
121      TCCGGGTTAC AGGAGCAGCG CAACCATTTG CAGGGCAAAC TGTCGGAGCT GCAGGTGGAG
181      CAGACATCCC TGGAGCCCCT CCAGGAGAGC CCCCGTCCCA CAGGTGTCTG GAAGTCCCGG
241      GAGGTAGCCA CCGAGGGCAT CCGTGGGCAC CGCAAAATGG TCCTCTACAC CCTGCGGGCA
301      CCACGAAGCC CCAAGATGGT GCAAGGGTCT GGCTGCTTTG GGAGGAAGAT GGACCGGATC
361      AGCTCCTCCA GTGGCCTGGG CTGCAAAGTG CTGAGGCGGC ATTAA

 

Quality Control:cDNA sequencing results is available upon request.