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Human NPPB Gene cDNA Clone (full-length ORF Clone)
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Human NPPB Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG13644-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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NPPB cDNA Clone Datasheet

 

NPPB cDNA Clone Product Information

Gene Bank Ref.ID: BC025785

cDNA Size:

405

Gene Synonym:

BNP;NPPB

Source: Human

NPPB cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens natriuretic peptide precursor B DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

NPPB cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NPPB cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NPPB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATCCCC AGACAGCACC TTCCCGGGCG CTCCTGCTCC TGCTCTTCTT GCATCTGGCT
61       TTCCTGGGAG GTCGTTCCCA CCCGCTGGGC AGCCCCGGTT CAGCCTCGGA CTTGGAAACG
121      TCCGGGTTAC AGGAGCAGCG CAACCATTTG CAGGGCAAAC TGTCGGAGCT GCAGGTGGAG
181      CAGACATCCC TGGAGCCCCT CCAGGAGAGC CCCCGTCCCA CAGGTGTCTG GAAGTCCCGG
241      GAGGTAGCCA CCGAGGGCAT CCGTGGGCAC CGCAAAATGG TCCTCTACAC CCTGCGGGCA
301      CCACGAAGCC CCAAGATGGT GCAAGGGTCT GGCTGCTTTG GGAGGAAGAT GGACCGGATC
361      AGCTCCTCCA GTGGCCTGGG CTGCAAAGTG CTGAGGCGGC ATTAA

 

Quality Control:cDNA sequencing results is available upon request.

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"