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Human NPM1 Gene cDNA Clone (full-length ORF Clone)
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Human NPM1 Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG12074-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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NPM1 cDNA Clone Datasheet

 

NPM1 cDNA Clone Product Information

Gene Bank Ref.ID: NM_002520

cDNA Size:

885

Gene Synonym:

B23, NPM

Source: Human

NPM1 cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens nucleophosmin (nucleolar phosphoprotein B23, numatrin) DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

NPM1 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NPM1 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

NPM1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAAGATT CGATGGACAT GGACATGAGC CCCCTGAGGC CCCAGAACTA TCTTTTCGGT
61       TGTGAACTAA AGGCCGACAA AGATTATCAC TTTAAGGTGG ATAATGATGA AAATGAGCAC
121      CAGTTATCTT TAAGAACGGT CAGTTTAGGG GCTGGTGCAA AGGATGAGTT GCACATTGTT
181      GAAGCAGAGG CAATGAATTA CGAAGGCAGT CCAATTAAAG TAACACTGGC AACTTTGAAA
241      ATGTCTGTAC AGCCAACGGT TTCCCTTGGG GGCTTTGAAA TAACACCACC AGTGGTCTTA
301      AGGTTGAAGT GTGGTTCAGG GCCAGTGCAT ATTAGTGGAC AGCACTTAGT AGCTGTGGAG
361      GAAGATGCAG AGTCAGAAGA TGAAGAGGAG GAGGATGTGA AACTCTTAAG TATATCTGGA
421      AAGCGGTCTG CCCCTGGAGG TGGTAGCAAG GTTCCACAGA AAAAAGTAAA ACTTGCTGCT
481      GATGAAGATG ATGACGATGA TGATGAAGAG GATGATGATG AAGATGATGA TGATGATGAT
541      TTTGATGATG AGGAAGCTGA AGAAAAAGCG CCAGTGAAGA AATCTATACG AGATACTCCA
601      GCCAAAAATG CACAAAAGTC AAATCAGAAT GGAAAAGACT CAAAACCATC ATCAACACCA
661      AGATCAAAAG GACAAGAATC CTTCAAGAAA CAGGAAAAAA CTCCTAAAAC ACCAAAAGGA
721      CCTAGTTCTG TAGAAGACAT TAAAGCAAAA ATGCAAGCAA GTATAGAAAA AGGTGGTTCT
781      CTTCCCAAAG TGGAAGCCAA ATTCATCAAT TATGTGAAGA ATTGCTTCCG GATGACTGAC
841      CAAGAGGCTA TTCAAGATCT CTGGCAGTGG AGGAAGTCTC TTTAA

 

Quality Control:cDNA sequencing results is available upon request.

 

NPM1 related areas, pathways, and other information

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"