Cynomolgus monkey NOV Gene cDNA Clone / ORF Clone

NOV cDNA Clone Datasheet

NOV cDNA Clone Product Information

• cDNA Size: 1074
• Gene Synonym: NOV
• Source: Cynomolgus monkey

NOV cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) nephroblastoma overexpressed gene DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 991 T/C resulting in the amino acid Cys substitution by Arg and 813 C/G not causing the amino acid variation.
  Please check the sequence information before order.

NOV cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

NOV cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

NOV cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAGAGTG TGCAGAGCAC GAGCTTTTGT CTCCGAAAGC AGTGCCTTTG CCTGACCTTC
61       CTGCTTCTCC ATCTCCTGGG ACAGGTCGCT GCGACTCAGC GCTGCCCTCC CCAGTGCCCG
121      GGCCAGTGCC CCACGACGCC GCCGACCTGC GCCCCCGGGG TGCGCGCGGT GCTGGACGGC
181      TGCTCCTGCT GTCCGGTGTG CGCCCGCCAG CGTGGCGAGA GCTGCTCAGA TCTGGAGCCA
241      TGCGACGAGA GCAGTGGCCT CTACTGTGAC CGCAGCGCAG ACCCCAGCAA CCAGACTGGC
301      ATCTGCATGG CGGTAGAGGG AGATAACTGT GTGTTCGATG GGGTCATCTA CCGCAGTGGA
361      GAGAAATTTC AGCCAAGCTG CAAATTCCAG TGCACCTGCA GAGATGGGCA GATTGGTTGT
421      GTGCCTCGCT GTCAGCTGGA CGTGCTACTG CCCGAGCCTA ACTGCCCAGC TCCAAGAAAA
481      GTTGAGGTGC CTGGGGAGTG CTGTGAAAAG TGGATCTGTG GCCCAGATGA GGAGGATTCA
541      CTGGGAGGCC TCACTCTTGC AGCTTACAGA CCAGAAGCCA CCCTAGGAGT AGAAGTCTCT
601      GACTCAAGTG TCAACTGCAT TGAACAGACC ACAGAGTGGA CGGCATGCTC TAAGAGCTGT
661      GGTATGGGGT TCTCCACCCG GGTCACCAAT AGGAACCGTC AATGTGAGAT GCTGAAACAG
721      ACTCGGCTCT GCATGGTGCG GCCCTGTGAA CAAGAGCCGG AGCAGCCAAC AGATAAGAAA
781      GGAAAAAAGT GTCTCCGCAC CAAGAAATCG CTGAAAGCCA TCCACCTGCA ATTCAAAAAC
841      TGCACCAGCC TGCACACCTA CAAGCCTAGG TTCTGTGGGG TCTGCAGTGA TGGTCGGTGC
901      TGCACTCCCC ACAATACCAA AACCATCCAG GCAGAGTTTC AGTGCTCCCC AGGGCAAATA
961      GTCAAGAAAC CAGTGATGGT CATTGGGACC CGCACCTGTC ACACCAACTG TCCTAAGAAT
1021     AATGAGGCCT TCCTCCAGGA GCTGGAGCTG AAGACTACCA GAGGGAAAAT GTAA

 

Quality Control:cDNA sequencing results is available upon request.