>Mouse NOTCH1 (aa 1-526) Protein (His Tag)
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Notch gene homolog 1 (Drosophila) Protein Datasheet
NOTCH1 Protein Price Inquiry ( Available Sizes )
NOTCH1 Protein Product Information
|Synonym :||RP23-306D20.12-001, 9930111A19Rik, Mis6, Tan1, lin-12|
|Protein Construction:||A DNA sequence encoding the mouse NOTCH1 (NP_032740.3) N-terminal fragment (Met 1-Gln 526) was expressed，with a C-terminal polyhistidine tag.|
|Expression Host:||Baculovirus-Insect cells|
NOTCH1 Protein QC Testing
|Purity:||>82 % as determined by SDS-PAGE.||SDS-PAGE:
|1. Measured by its binding ability in a functional ELISA.
2. Immobilized human DLL4 (cat: 10171-H02H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 40 ng/mL.
3. Immobilized mouse DLL4-his (Cat:50640-M08H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 30 ng/mL.
|Endotoxin:||< 1.0 EU per μg of the protein as determined by the LAL method|
|Stability:||Samples are stable for up to twelve months from date of receipt at -70℃|
|Predicted N terminal:||Ala 19|
|Molecular Mass:||The secreted recombinant mouse NOTCH1 (aa 1-526) consists of 518 amino acids and has a calculated molecular mass of 55 kDa. It migrates as an approximately 80 KDa band in SDS-PAGE under reducing conditions.|
|Formulation:||Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
NOTCH1 Protein Usage Guide
|Storage:||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution:||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
NOTCH1 Protein Related Products & Topics
Stem Cell>>Notch Signaling Pathway>> Notch1
NOTCH1 Protein Description
- Li L. et al., 1997, Nat Genet. 16 (3): 243-51.
- Radtke F. et al., 1999, Immunity. 10 (5): 547-58.
- Gaiano N. et al., 2000, Neuron. 26 (2): 395-404.