Mouse NOTCH1 (aa 1-526) Protein (His Tag)

Notch gene homolog 1 (Drosophila) Protein Datasheet

NOTCH1 Protein Product Information

• Synonym: RP23-306D20.12-001, 9930111A19Rik, Mis6, Tan1, lin-12
• Protein Construction: A DNA sequence encoding the mouse NOTCH1 (NP_032740.3) N-terminal fragment (Met 1-Gln 526) was expressed，with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Baculovirus-Insect cells

NOTCH1 Protein QC Testing

• Purity: >82 % as determined by SDS-PAGE.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  2. Immobilized human DLL4 (cat: 10171-H02H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC₅₀ of biotinylated mouse NOTCH1-his is 40 ng/mL.
  3. Immobilized mouse DLL4-his (Cat:50640-M08H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC₅₀ of biotinylated mouse NOTCH1-his is 30 ng/mL.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 19
• Molecular Mass: The secreted recombinant mouse NOTCH1 (aa 1-526) consists of 518 amino acids and has a calculated molecular mass of 55 kDa. It migrates as an approximately 80 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: NOTCH1 protein

NOTCH1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

NOTCH1 Protein Description

Notch1, translocation-associated (Drosophila), also known as NOTCH homolog 1, is a member of the Notch family. This type-1 transmembrane protein share structure characteristic including an extracellular domain consisting of multiple epidermal growth factor-like(EGF) repeats, and an intracellular domain consisting of different domain types. Notch1 plays an obligatory and selective role in T cell lineage induction. Notch1 deficiency in skin and in primary keratinocytes results in increased and sustained expression of Gli2, causing the development of basal-cell carcinoma–like tumors. Furthermore, Notch1 inactivation in the epidermis results in derepressed beta-catenin signaling in cells that should normally undergo differentiation. Enhanced beta-catenin signaling can be reversed by re-introduction of a dominant active form of the Notch1 receptor. This leads to a reduction in the signaling-competent pool of beta-catenin, indicating that Notch1 can inhibit beta-catenin-mediated signaling. Our results indicate that Notch1 functions as a tumor-suppressor gene in mammalian skin.

References

1. Li L. et al., 1997, Nat Genet. 16 (3): 243-51.
2. Radtke F. et al., 1999, Immunity. 10 (5): 547-58.
3. Gaiano N. et al., 2000, Neuron. 26 (2): 395-404.