Anti-NME1 Antibody

Cat: 11615-RP02
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Anti-NME1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-NME1 Antibody
Validated applications
Species reactivity
Reacts with: Human
Human NME1
Recombinant Human NME1 protein (Catalog#11615-H07E)
Produced in rabbits immunized with purified, recombinant Human NME1 (rh NME1; Catalog#11615-H07E; NP_000260.1; Ala 2-Glu 152). NME1 IgG was purified by human NME1 affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-NME1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-NME1 Antibody (Rabbit Polyclonal antibody) Images
Human NME1/NDKA Immunoprecipitation(IP) 8099

NME1 was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

Lane C:0.5 mg Jurkat Whole Cell Lysate

Lane D:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-NME1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-NME1 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size: 18 kDa

Human NME1/NDKA Western blot (WB) 13427

Anti-NME1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: Hela Whole Cell Lysate

Lane D: MCF7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:17 kDa

Observed band size:21 kDa

(We are unsure as to the identity of these extra bands.)

Anti-NME1 Antibody Alternative Names
Anti-AWD Antibody;Anti-GAAD Antibody;Anti-NB Antibody;Anti-NBS Antibody;Anti-NDKA Antibody;Anti-NDPK-A Antibody;Anti-NDPKA Antibody;Anti-NM23 Antibody;Anti-NM23-H1 Antibody
NME1 Background Information

NME1, also known as Nucleoside Diphosphate Kinase A (NDK-A), or NM23-H1, belongs to the NDK family. NM23-H1 is known to have a metastasis suppressive activity in many tumor cells. Recent studies have shown that the interacting proteins with NM23-H1 which mediate the cell proliferation, may act as modulators of the metastasis suppressor activity. The interacting proteins with NM23-H1 can be classified into 3 groups. The first group of proteins can be classified as upstream kinases of NM23-H1 such as CKI and Aurora-A/STK15. The second group of proteins acts as downstream effectors for the regulation of specific gene transcriptions, GTP-binding protein functions, and signal transduction in Erk signal cascade. The third group of proteins can be classified as bi-directionally influencing binding partners of NM23-H1. As a result, the interactions with NM23-H1 and binding partners have implications in the biochemical characterization involved in metastasis and tumorigenesis. NDKA is increased in human postmortem cerebrospinal fluid (CSF), a model of global brain insult, suggesting that measurement in CSF and, more importantly, in plasma may be useful as a biomarker of stroke. Additionally, NM23-H1 significantly reduces metastasis without effects on primary tumor size and was the first discovered metastasis suppressor gene.

Full Name
NME/NM23 nucleoside diphosphate kinase 1
  • Allard L, et al. (2005) PARK7 and nucleoside diphosphate kinase A as plasma markers for the early diagnosis of stroke. Clin Chem. 51(11): 2043-51.
  • Steeg PS, et al. (2008) Clinical-translational approaches to the Nm23-H1 metastasis suppressor. Clin Cancer Res. 14(16): 5006-12.
  • Kim HD, et al. (2009) Regulators affecting the metastasis suppressor activity of Nm23-H1. Mol Cell Biochem. 329(1-2): 167-73.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-NME1 Antibody Rabbit Polyclonal IHC-P 106374-T08
    Anti-NME1 Antibody Rabbit Polyclonal ELISA 11615-T16
    Anti-NME1 Antibody Rabbit Monoclonal WB 11615-R011
    Anti-NME1 Antibody Rabbit Monoclonal ELISA 11615-R001
    Anti-NME1 Antibody Rabbit Polyclonal WB,ICC/IF,IF,IP 101691-T38
    Anti-NME1 Antibody Mouse Monoclonal FCM,ICC/IF,IF 11615-MM01
    Anti-NME1 Antibody (PE) Mouse Monoclonal FCM 11615-MM01-P
    Anti-NME1 Antibody Rabbit Monoclonal IHC-P 11615-R084
    Anti-NME1 Antibody Rabbit Polyclonal ELISA 11615-RP01
    Anti-NME1 Antibody (FITC) Mouse Monoclonal FCM 11615-MM01-F
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