Anti-NME1 Antibody

Cat: 11615-RP02
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All NME1 Reagents
Anti-NME1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-NME1 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human NME1
Immunogen
Recombinant Human NME1 protein (Catalog#11615-H07E)
Preparation
Produced in rabbits immunized with purified, recombinant Human NME1 (rh NME1; Catalog#11615-H07E; NP_000260.1; Ala 2-Glu 152). NME1 IgG was purified by human NME1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-NME1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-NME1 Antibody (Rabbit Polyclonal antibody) Images
Human NME1/NDKA Immunoprecipitation(IP) 8099

NME1 was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

Lane C:0.5 mg Jurkat Whole Cell Lysate

Lane D:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-NME1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-NME1 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size: 18 kDa

Human NME1/NDKA Western blot (WB) 13427

Anti-NME1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: Hela Whole Cell Lysate

Lane D: MCF7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:17 kDa

Observed band size:21 kDa

(We are unsure as to the identity of these extra bands.)

Anti-NME1 Antibody Alternative Names
Anti-AWD Antibody;Anti-GAAD Antibody;Anti-NB Antibody;Anti-NBS Antibody;Anti-NDKA Antibody;Anti-NDPK-A Antibody;Anti-NDPKA Antibody;Anti-NM23 Antibody;Anti-NM23-H1 Antibody
NME1 Background Information

NME1, also known as Nucleoside Diphosphate Kinase A (NDK-A), or NM23-H1, belongs to the NDK family. NM23-H1 is known to have a metastasis suppressive activity in many tumor cells. Recent studies have shown that the interacting proteins with NM23-H1 which mediate the cell proliferation, may act as modulators of the metastasis suppressor activity. The interacting proteins with NM23-H1 can be classified into 3 groups. The first group of proteins can be classified as upstream kinases of NM23-H1 such as CKI and Aurora-A/STK15. The second group of proteins acts as downstream effectors for the regulation of specific gene transcriptions, GTP-binding protein functions, and signal transduction in Erk signal cascade. The third group of proteins can be classified as bi-directionally influencing binding partners of NM23-H1. As a result, the interactions with NM23-H1 and binding partners have implications in the biochemical characterization involved in metastasis and tumorigenesis. NDKA is increased in human postmortem cerebrospinal fluid (CSF), a model of global brain insult, suggesting that measurement in CSF and, more importantly, in plasma may be useful as a biomarker of stroke. Additionally, NM23-H1 significantly reduces metastasis without effects on primary tumor size and was the first discovered metastasis suppressor gene.

Full Name
NME/NM23 nucleoside diphosphate kinase 1
References
  • Allard L, et al. (2005) PARK7 and nucleoside diphosphate kinase A as plasma markers for the early diagnosis of stroke. Clin Chem. 51(11): 2043-51.
  • Steeg PS, et al. (2008) Clinical-translational approaches to the Nm23-H1 metastasis suppressor. Clin Cancer Res. 14(16): 5006-12.
  • Kim HD, et al. (2009) Regulators affecting the metastasis suppressor activity of Nm23-H1. Mol Cell Biochem. 329(1-2): 167-73.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-NME1 Antibody Rabbit Polyclonal IHC-P 106374-T08
    Anti-NME1 Antibody Rabbit Polyclonal ELISA 11615-T16
    Anti-NME1 Antibody Rabbit Monoclonal WB 11615-R011
    Anti-NME1 Antibody Rabbit Monoclonal ELISA 11615-R001
    Anti-NME1 Antibody Rabbit Polyclonal WB,ICC/IF,IF,IP 101691-T38
    Anti-NME1 Antibody Mouse Monoclonal FCM,ICC/IF,IF 11615-MM01
    Anti-NME1 Antibody (PE) Mouse Monoclonal FCM 11615-MM01-P
    Anti-NME1 Antibody Rabbit Monoclonal IHC-P 11615-R084
    Anti-NME1 Antibody Rabbit Polyclonal ELISA 11615-RP01
    Anti-NME1 Antibody (FITC) Mouse Monoclonal FCM 11615-MM01-F
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